##gff-version 3
##sequence-region BBa_S03913 1 1081
BBa_S03913	.	engineered_region	168	1081	.	+	0	ID=BBa_S03903;Name=BBa_S03903
BBa_S03913	.	ribosome_entry_site	168	179	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03903
BBa_S03913	.	conserved_region	172	175	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03913	.	terminator	953	1032	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03903
BBa_S03913	.	stem_loop	964	1007	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03913	.	engineered_region	953	1032	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03913	.	terminator	1041	1081	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03903
BBa_S03913	.	polyA_site	1068	1081	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03913	.	stem_loop	1048	1067	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03913	.	engineered_region	1041	1081	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03913	.	stop_codon	1074	1074	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03913	.	engineered_region	186	944	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_S03903
BBa_S03913	.	tag	900	938	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_S03913	.	engineered_region	186	905	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_S03913	.	start_codon	186	188	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_S03913	.	stop_codon	939	944	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_S03913	.	promoter	1	159	.	+	0	ID=BBa_I715053;Name=BBa_I715053
BBa_S03913	.	sequence_feature	152	159	.	+	0	ID=annotation1958530;Name=Poly T;Parent=BBa_I715053
BBa_S03913	.	ribosome_entry_site	5	13	.	+	0	ID=annotation1958526;Name=predicted RBS;Parent=BBa_I715053
BBa_S03913	.	conserved_region	47	52	.	+	0	ID=annotation1958528;Name=tandem trp codons;Parent=BBa_I715053
BBa_S03913	.	CDS	20	64	.	+	0	ID=annotation1958527;Name=leader peptide;Parent=BBa_I715053
BBa_S03913	.	stop_codon	62	64	.	+	0	ID=annotation1958529;Name=stop;Parent=BBa_I715053
BBa_S03913	.	sequence_feature	91	116	.	+	0	ID=annotation1958531;Name=hixC;Parent=BBa_I715053
BBa_S03913	.	start_codon	20	22	.	+	0	ID=annotation1958525;Name=start;Parent=BBa_I715053
>BBa_S03913
cgtaaaaagggtatcgacaatgaaagcaattttcgtactgaaaggttggtggcgcacttcctgaaacgggcagtgtatt
caccatgcgtattatcaaaaaccatggtttttgataaagcaatcagatacccagcccgcctaatgagcgggcttttttt
ttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaat
tagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccct
taaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgc
tttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaa
ctatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaat
cgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaat
gtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcg
ttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtc
cacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggg
attacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata