##gff-version 3
##sequence-region BBa_S03871 1 1772
BBa_S03871	.	engineered_region	1	828	.	+	0	ID=BBa_S03870;Name=BBa_S03870
BBa_S03871	.	mature_transcript_region	63	102	.	+	0	ID=BBa_J01010;Name=BBa_J01010;Parent=BBa_S03870
BBa_S03871	.	CDS	109	828	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03870
BBa_S03871	.	CDS	109	828	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S03871	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03870
BBa_S03871	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03871	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03871	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03871	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03871	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03871	.	engineered_region	837	1772	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_S03871	.	terminator	1732	1772	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_S03871	.	stop_codon	1765	1765	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03871	.	polyA_site	1759	1772	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03871	.	stem_loop	1739	1758	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03871	.	engineered_region	1732	1772	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03871	.	terminator	1644	1723	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_S03871	.	engineered_region	1644	1723	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03871	.	stem_loop	1655	1698	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03871	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_S03871	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03871	.	CDS	855	1610	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_S03871	.	point_mutation	1346	1346	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S03871	.	engineered_tag	1611	1635	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S03871	.	engineered_region	855	856	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S03871	.	engineered_region	855	1610	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S03871	.	CDS	855	1604	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S03871	.	point_mutation	1028	1028	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
>BBa_S03871
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctctt
ggatttgggtattaaagaggagatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatgaaaaacataaat
gccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgata
tgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaat
cctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattat
tctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaatta
aagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttag
ttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcct
tctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaat
gtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcacttt
ccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagca
attgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata