##gff-version 3
##sequence-region BBa_S03751 1 5216
BBa_S03751	.	engineered_region	1	875	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_S03751	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_S03751	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03751	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_S03751	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03751	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03751	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03751	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03751	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_S03751	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03751	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03751	.	CDS	19	738	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_S03751	.	CDS	19	738	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S03751	.	engineered_region	884	5216	.	+	0	ID=BBa_M30109;Name=BBa_M30109
BBa_S03751	.	CDS	1817	2566	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_M30109
BBa_S03751	.	engineered_tag	2567	2591	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_S03751	.	CDS	1817	2566	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_S03751	.	ribosome_entry_site	1799	1810	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_S03751	.	conserved_region	1803	1806	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03751	.	terminator	5088	5167	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_S03751	.	engineered_region	5088	5167	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03751	.	stem_loop	5099	5142	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03751	.	promoter	884	1013	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_M30109
BBa_S03751	.	ribosome_entry_site	2799	2810	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_S03751	.	conserved_region	2803	2806	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03751	.	CDS	1040	1765	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_M30109
BBa_S03751	.	engineered_tag	1766	1790	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_S03751	.	terminator	2600	2679	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_S03751	.	engineered_region	2600	2679	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03751	.	stem_loop	2611	2654	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03751	.	terminator	5176	5216	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_S03751	.	stem_loop	5183	5202	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03751	.	stop_codon	5209	5209	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03751	.	engineered_region	5176	5216	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03751	.	polyA_site	5203	5216	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03751	.	terminator	2688	2728	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_S03751	.	stem_loop	2695	2714	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03751	.	stop_codon	2721	2721	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03751	.	engineered_region	2688	2728	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03751	.	polyA_site	2715	2728	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03751	.	CDS	2817	5054	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_M30109
BBa_S03751	.	engineered_tag	5055	5079	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_S03751	.	CDS	2817	5054	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_S03751	.	promoter	2737	2790	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_M30109
BBa_S03751	.	promoter	2779	2784	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03751	.	non_covalent_binding_site	2762	2780	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03751	.	non_covalent_binding_site	2737	2755	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03751	.	promoter	2756	2761	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03751	.	engineered_region	2737	2790	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03751	.	ribosome_entry_site	1022	1033	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_S03751	.	conserved_region	1026	1029	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03751
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcg
gatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagag
gagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacg
tcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgt
ctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaac
cgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctg
gccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgtta
cctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagct
ttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattg
accaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttga
aggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagtt
ggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactaga
tggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggc
gatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctg
gaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaagg
tgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacat
tgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcg
taccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatatttt
ctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgca
aattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcat
tactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggt
atatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccc
tatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgacc
aatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgca
agaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggc
agaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcc
tcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtga
cggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaac
gctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatga
ctggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatga
tatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaacccc
attcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtgg
atttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtcttt
aaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattcccttt
gaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcg
attaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggatt
aactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgatttta
gtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttct
tcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccat
tgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgct
tacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgac
tccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcg
ccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagt
cacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaat
cgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgcc
gatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaacc
gcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacg
ccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatga
cggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagc
ggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagc
ggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataact
ctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta