##gff-version 3 ##sequence-region BBa_S03596 1 2899 BBa_S03596 . promoter 1260 1389 . + 0 ID=BBa_I13453;Name=BBa_I13453 BBa_S03596 . terminator 2774 2814 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_S03596 . engineered_region 2774 2814 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_S03596 . stop_codon 2807 2807 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_S03596 . polyA_site 2801 2814 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_S03596 . stem_loop 2781 2800 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_S03596 . sequence_feature 2652 2677 . + 0 ID=BBa_J44000;Name=BBa_J44000 BBa_S03596 . engineered_region 1 1114 . + 0 ID=BBa_I13458;Name=BBa_I13458 BBa_S03596 . sequence_feature 2823 2899 . + 0 ID=BBa_J3101;Name=BBa_J3101 BBa_S03596 . sequence_feature 2851 2851 . + 0 ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101 BBa_S03596 . non_covalent_binding_site 2877 2891 . + 0 ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101 BBa_S03596 . sequence_feature 2873 2873 . + 0 ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101 BBa_S03596 . non_covalent_binding_site 2828 2843 . + 0 ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101 BBa_S03596 . sequence_feature 2899 2899 . + 0 ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101 BBa_S03596 . sequence_feature 2823 2899 . + 0 ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101 BBa_S03596 . sequence_feature 2873 2878 . + 0 ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101 BBa_S03596 . terminator 1123 1202 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_S03596 . engineered_region 1123 1202 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_S03596 . stem_loop 1134 1177 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_S03596 . terminator 1211 1251 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_S03596 . engineered_region 1211 1251 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_S03596 . stop_codon 1244 1244 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_S03596 . polyA_site 1238 1251 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_S03596 . stem_loop 1218 1237 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_S03596 . CDS 1453 2643 . + 0 ID=BBa_J31007;Name=BBa_J31007 BBa_S03596 . CDS 1453 2643 . + 0 ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007 BBa_S03596 . primer_binding_site 1453 1476 . + 0 ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007 BBa_S03596 . stop_codon 2641 2643 . + 0 ID=annotation1910573;Name=stop;Parent=BBa_J31007 BBa_S03596 . primer_binding_site 2624 2643 . + 0 ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007 BBa_S03596 . ribosome_entry_site 1432 1446 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_S03596 . ribosome_entry_site 1432 1446 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_S03596 . engineered_region 1432 1446 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_S03596 . ribosome_entry_site 1439 1443 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_S03596 . sequence_feature 1398 1423 . + 0 ID=BBa_J44000;Name=BBa_J44000 BBa_S03596 . terminator 2686 2765 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_S03596 . engineered_region 2686 2765 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_S03596 . stem_loop 2697 2740 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 >BBa_S03596 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac tttgctattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacatt gattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttt tatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgata atactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctgg atgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccag tcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgc tttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcc tgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccga catcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggcccc gtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctac tactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcag ctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacag gtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcgg tattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccat tatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccatt atgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatc agggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgattta tgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttg cgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaaccatggtttttgataatactagagc caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgacca aaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg