##gff-version 3
##sequence-region BBa_S03564 1 2654
BBa_S03564	.	ribosome_entry_site	1221	1235	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03564	.	ribosome_entry_site	1228	1232	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03564	.	engineered_region	1221	1235	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03564	.	ribosome_entry_site	1221	1235	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03564	.	engineered_region	1	1212	.	+	0	ID=BBa_S03562;Name=BBa_S03562
BBa_S03564	.	CDS	22	1212	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03562
BBa_S03564	.	primer_binding_site	1193	1212	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03564	.	primer_binding_site	22	45	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03564	.	CDS	22	1212	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03564	.	stop_codon	1210	1212	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03564	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_S03562
BBa_S03564	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03564	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03564	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03564	.	terminator	2441	2569	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S03564	.	terminator	2441	2520	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03564	.	engineered_region	2441	2520	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03564	.	stem_loop	2452	2495	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03564	.	terminator	2529	2569	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03564	.	stop_codon	2562	2562	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03564	.	polyA_site	2556	2569	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03564	.	stem_loop	2536	2555	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03564	.	engineered_region	2529	2569	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03564	.	CDS	1242	2432	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03564	.	primer_binding_site	2413	2432	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03564	.	primer_binding_site	1242	1265	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03564	.	CDS	1242	2432	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03564	.	stop_codon	2430	2432	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03564	.	sequence_feature	2578	2654	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03564	.	sequence_feature	2628	2628	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03564	.	non_covalent_binding_site	2632	2646	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03564	.	sequence_feature	2654	2654	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03564	.	sequence_feature	2606	2606	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03564	.	non_covalent_binding_site	2583	2598	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03564	.	sequence_feature	2578	2654	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03564	.	sequence_feature	2628	2633	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
>BBa_S03564
attaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtag
gcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatgg
cgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgc
cgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcc
tctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccga
tggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggg
ggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggct
gcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccg
gtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggca
gcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaa
tcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccgg
catggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattctt
ctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagc
ttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctc
ggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggt
gcatggagccgggccacctcgacctaatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcat
cgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggat
atcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcac
ccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgacta
cgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggt
gcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtt
tcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggc
ggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatg
cccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtct
tctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgc
gacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccacc
aaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcga
cgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgct
gtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcatt
ggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccc
tataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatattg
atttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg