##gff-version 3
##sequence-region BBa_S03551 1 2348
BBa_S03551	.	engineered_region	1471	2348	.	+	0	ID=BBa_S03540;Name=BBa_S03540
BBa_S03551	.	ribosome_entry_site	1471	1482	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03540
BBa_S03551	.	conserved_region	1475	1478	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03551	.	terminator	2220	2299	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03540
BBa_S03551	.	stem_loop	2231	2274	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03551	.	engineered_region	2220	2299	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03551	.	engineered_region	1489	2211	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_S03540
BBa_S03551	.	CDS	1489	2196	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_S03551	.	tag	2197	2205	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_S03551	.	sequence_alteration	1840	1840	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_S03551	.	terminator	2308	2348	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03540
BBa_S03551	.	engineered_region	2308	2348	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03551	.	stop_codon	2341	2341	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03551	.	stem_loop	2315	2334	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03551	.	polyA_site	2335	2348	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03551	.	engineered_region	1	1462	.	+	0	ID=BBa_S03145;Name=BBa_S03145
BBa_S03551	.	CDS	19	768	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_S03145
BBa_S03551	.	tag	730	762	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S03551	.	CDS	22	729	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S03551	.	engineered_tag	769	793	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S03551	.	ribosome_entry_site	802	813	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03145
BBa_S03551	.	conserved_region	806	809	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03551	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03145
BBa_S03551	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03551	.	CDS	820	1437	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_S03145
BBa_S03551	.	engineered_region	820	1437	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03551	.	tag	1399	1430	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03551	.	engineered_tag	1438	1462	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03551	.	CDS	820	1398	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
>BBa_S03551
aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag
caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg
cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagc
gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa
gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga
tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc
gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg
atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca
accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg
cctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagat
cgctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggagga
gtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacctt
gaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggc
gtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaaga
tcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattaca
atgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaa
tagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaa
atcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagg
gcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacga
gttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggc
cccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgaca
tccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggt
gaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctcc
gacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctga
agggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaa
gaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatc
gtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata