##gff-version 3
##sequence-region BBa_S03516 1 214
BBa_S03516	.	sequence_feature	138	214	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03516	.	non_covalent_binding_site	143	158	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03516	.	sequence_feature	166	166	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03516	.	non_covalent_binding_site	192	206	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03516	.	sequence_feature	188	188	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03516	.	sequence_feature	188	193	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03516	.	sequence_feature	138	214	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03516	.	sequence_feature	214	214	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03516	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03516	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03516	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03516	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03516	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03516	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03516	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03516	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S03516
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgacc
aaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg