##gff-version 3
##sequence-region BBa_S03448 1 1333
BBa_S03448	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S03448	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S03448	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S03448	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S03448	.	engineered_region	230	1196	.	+	0	ID=BBa_P1003;Name=BBa_P1003
BBa_S03448	.	CDS	378	1196	.	+	0	ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003
BBa_S03448	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S03448	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03448	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03448	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03448	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03448	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03448	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03448	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03448	.	terminator	1205	1333	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S03448	.	terminator	1293	1333	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03448	.	engineered_region	1293	1333	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03448	.	stem_loop	1300	1319	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03448	.	stop_codon	1326	1326	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03448	.	polyA_site	1320	1333	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03448	.	terminator	1205	1284	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03448	.	engineered_region	1205	1284	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03448	.	stem_loop	1216	1259	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_S03448
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagagctgatcct
tcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataa
aaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgg
gaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatg
tcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaagg
tagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaag
cattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaag
aatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaa
ttgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagt
gattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccgg
attcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgt
tggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcatta
cagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagt
ttttctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata