##gff-version 3
##sequence-region BBa_S03431 1 1331
BBa_S03431	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03431	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03431	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03431	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03431	.	CDS	22	1149	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S03431	.	engineered_region	22	1149	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S03431	.	CDS	22	1149	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S03431	.	engineered_tag	1150	1174	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S03431	.	tag	1111	1149	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S03431	.	terminator	1237	1331	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_S03431	.	terminator	1286	1331	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_S03431	.	engineered_region	1286	1331	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_S03431	.	stem_loop	1298	1320	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_S03431	.	terminator	1237	1277	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_S03431	.	polyA_site	1264	1277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03431	.	engineered_region	1237	1277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03431	.	stop_codon	1270	1270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03431	.	stem_loop	1244	1263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03431	.	terminator	1183	1228	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_S03431	.	engineered_region	1183	1228	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_S03431	.	stem_loop	1195	1217	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_S03431
attaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctt
atcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc
ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcc
agtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtgg
tgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggct
gatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttctt
gatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctgg
tcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctg
gcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaa
caaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaa
tgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctc
atgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaa
ctctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaga
gaatataaaaagccagattattaatccggcttttttattattttactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt