##gff-version 3
##sequence-region BBa_S03423 1 2455
BBa_S03423	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03423	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03423	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03423	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03423	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03423	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03423	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03423	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03423	.	promoter	138	191	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S03423	.	promoter	180	185	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03423	.	promoter	157	162	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03423	.	engineered_region	138	191	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03423	.	non_covalent_binding_site	163	181	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03423	.	non_covalent_binding_site	138	156	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03423	.	CDS	218	2455	.	+	0	ID=BBa_I15010;Name=BBa_I15010
BBa_S03423	.	CDS	218	2455	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_S03423	.	engineered_tag	2456	2480	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_S03423	.	ribosome_entry_site	200	211	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03423	.	conserved_region	204	207	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03423
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccaccaccgtacaact
cagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtg
gtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatt
tgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaat
cagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgc
aacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcaca
tggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccg
ccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaa
cgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatcc
acaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccg
agcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtagga
gcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaa
ttccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacgga
aaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtc
gaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaat
tgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaaga
cgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggcc
attcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaa
atcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaat
tgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctc
attttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgg
gggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatct
ggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcag
gagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaa
ttgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggt
ggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtg
gaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgca
ccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcac
cagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaaggg
taataactctgatagtgctagtgtagatctc