##gff-version 3
##sequence-region BBa_S03329 1 2125
BBa_S03329	.	engineered_region	1227	1988	.	+	0	ID=BBa_E0034;Name=BBa_E0034
BBa_S03329	.	tag	1944	1982	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_S03329	.	stop_codon	1983	1988	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_S03329	.	terminator	2085	2125	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03329	.	stem_loop	2092	2111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03329	.	polyA_site	2112	2125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03329	.	stop_codon	2118	2118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03329	.	engineered_region	2085	2125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03329	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03329	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03329	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03329	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03329	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03329	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S03329	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S03329	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S03329	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S03329	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S03329	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S03329	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S03329	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_S03329	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S03329	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S03329	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S03329	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S03329	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S03329	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S03329	.	promoter	1104	1200	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S03329	.	promoter	1174	1179	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S03329	.	engineered_region	1104	1200	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S03329	.	promoter	1161	1161	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S03329	.	non_covalent_binding_site	1160	1176	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S03329	.	promoter	1150	1155	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S03329	.	non_covalent_binding_site	1184	1200	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S03329	.	promoter	1197	1200	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S03329	.	non_covalent_binding_site	1109	1128	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S03329	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03329	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03329	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03329	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03329	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03329	.	terminator	1997	2076	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03329	.	stem_loop	2008	2051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03329	.	engineered_region	1997	2076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03329	.	ribosome_entry_site	1209	1220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03329	.	conserved_region	1213	1216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03329
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaa
gcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatttta
cctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggt
gcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacc
tacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcg
gctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaagg
ctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtaca
actacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaa
catcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgccc
gacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagt
tcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgc
tgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata