##gff-version 3
##sequence-region BBa_S03187 1 1978
BBa_S03187	.	engineered_region	1471	1978	.	+	0	ID=BBa_E0433;Name=BBa_E0433
BBa_S03187	.	engineered_region	1489	1841	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_E0433
BBa_S03187	.	promoter	1661	1679	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_S03187	.	sequence_alteration	1566	1566	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_S03187	.	sequence_alteration	1599	1599	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_S03187	.	promoter	1514	1523	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_S03187	.	sequence_alteration	1569	1569	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_S03187	.	sequence_alteration	1596	1596	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_S03187	.	sequence_alteration	1575	1575	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_S03187	.	CDS	1489	1836	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_S03187	.	sequence_feature	1687	1836	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_S03187	.	sequence_feature	1489	1503	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_S03187	.	ribosome_entry_site	1471	1482	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0433
BBa_S03187	.	conserved_region	1475	1478	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03187	.	terminator	1850	1978	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0433
BBa_S03187	.	terminator	1850	1929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03187	.	engineered_region	1850	1929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03187	.	stem_loop	1861	1904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03187	.	terminator	1938	1978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03187	.	stop_codon	1971	1971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03187	.	polyA_site	1965	1978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03187	.	stem_loop	1945	1964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03187	.	engineered_region	1938	1978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03187	.	CDS	820	1437	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S03187	.	CDS	820	1398	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S03187	.	engineered_tag	1438	1462	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03187	.	engineered_region	820	1437	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03187	.	tag	1399	1430	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03187	.	ribosome_entry_site	802	813	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03187	.	conserved_region	806	809	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03187	.	CDS	19	793	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S03187	.	engineered_tag	769	793	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S03187	.	CDS	22	729	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S03187	.	tag	730	762	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S03187	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03187	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03187
aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag
caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg
cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagc
gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa
gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga
tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc
gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg
atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca
accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg
cctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagat
cgctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggagga
gtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacctt
gaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggc
gtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaaga
tcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattaca
atgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaa
tagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaa
atcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgaccatgatta
cgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagt
ggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcg
ccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttaccc
aacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca
acagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata