##gff-version 3
##sequence-region BBa_S01932 1 3108
BBa_S01932	.	terminator	808	887	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01932	.	stem_loop	819	862	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01932	.	engineered_region	808	887	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01932	.	terminator	2081	2121	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01932	.	stem_loop	2088	2107	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01932	.	stop_codon	2114	2114	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01932	.	polyA_site	2108	2121	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01932	.	engineered_region	2081	2121	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01932	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01932	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01932	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01932	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01932	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01932	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01932	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01932	.	CDS	1171	1959	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S01932	.	CDS	1171	1920	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01932	.	tag	1921	1953	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01932	.	engineered_region	1171	1959	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01932	.	engineered_tag	1960	1984	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01932	.	start_codon	1171	1173	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01932	.	stop_codon	1954	1959	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01932	.	promoter	2130	2183	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01932	.	engineered_region	2130	2183	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01932	.	promoter	2149	2154	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01932	.	non_covalent_binding_site	2130	2148	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01932	.	promoter	2172	2177	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01932	.	non_covalent_binding_site	2155	2173	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01932	.	ribosome_entry_site	1153	1164	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01932	.	conserved_region	1157	1160	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01932	.	ribosome_entry_site	2192	2203	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01932	.	conserved_region	2196	2199	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01932	.	engineered_region	2210	2971	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01932	.	sequence_alteration	2451	2451	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01932	.	engineered_region	2210	2971	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01932	.	tag	2927	2965	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01932	.	stop_codon	2966	2971	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01932	.	CDS	2210	2971	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01932	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01932	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01932	.	terminator	3068	3108	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01932	.	stem_loop	3075	3094	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01932	.	stop_codon	3101	3101	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01932	.	polyA_site	3095	3108	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01932	.	engineered_region	3068	3108	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01932	.	promoter	945	1144	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01932	.	promoter	1081	1086	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01932	.	CDS	945	1032	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01932	.	engineered_region	945	1144	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01932	.	promoter	1105	1110	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01932	.	non_covalent_binding_site	1110	1144	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01932	.	start_codon	1117	1117	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01932	.	non_covalent_binding_site	1033	1070	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01932	.	terminator	2980	3059	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01932	.	stem_loop	2991	3034	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01932	.	engineered_region	2980	3059	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01932	.	terminator	1993	2072	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01932	.	stem_loop	2004	2047	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01932	.	engineered_region	1993	2072	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01932	.	terminator	896	936	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01932	.	stem_loop	903	922	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01932	.	stop_codon	929	929	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01932	.	polyA_site	923	936	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01932	.	engineered_region	896	936	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S01932
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaat
acgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc
agtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgta
tgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacagtaaagaag
ctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattag
acttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagt
taataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtg
aatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcag
gaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaata
tttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaatta
ttgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacga
ttgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttc
aattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaagggga
tgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagt
gtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatg
gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagt
tcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagct
gcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaag
ctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaact
acaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagga
ggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaag
aacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcaga
acacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccc
caacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata