##gff-version 3
##sequence-region BBa_S01930 1 3148
BBa_S01930	.	promoter	807	957	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_S01930	.	promoter	895	900	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S01930	.	promoter	921	928	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S01930	.	non_covalent_binding_site	936	955	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S01930	.	non_covalent_binding_site	807	826	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S01930	.	engineered_region	807	957	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S01930	.	start_codon	934	934	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S01930	.	promoter	3095	3148	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01930	.	engineered_region	3095	3148	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01930	.	promoter	3114	3119	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01930	.	non_covalent_binding_site	3095	3113	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01930	.	promoter	3137	3142	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01930	.	non_covalent_binding_site	3120	3138	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01930	.	promoter	1910	2109	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01930	.	promoter	2046	2051	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01930	.	CDS	1910	1997	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01930	.	engineered_region	1910	2109	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01930	.	promoter	2070	2075	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01930	.	non_covalent_binding_site	2075	2109	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01930	.	start_codon	2082	2082	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01930	.	non_covalent_binding_site	1998	2035	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01930	.	terminator	670	749	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01930	.	stem_loop	681	724	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01930	.	engineered_region	670	749	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01930	.	terminator	2958	3037	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01930	.	stem_loop	2969	3012	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01930	.	engineered_region	2958	3037	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01930	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01930	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01930	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01930	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01930	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01930	.	CDS	2136	2924	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S01930	.	CDS	2136	2885	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01930	.	tag	2886	2918	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01930	.	engineered_region	2136	2924	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01930	.	engineered_tag	2925	2949	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01930	.	start_codon	2136	2138	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01930	.	stop_codon	2919	2924	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01930	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01930	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01930	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01930	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01930	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01930	.	terminator	1773	1852	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01930	.	stem_loop	1784	1827	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01930	.	engineered_region	1773	1852	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01930	.	ribosome_entry_site	966	977	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01930	.	conserved_region	970	973	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01930	.	ribosome_entry_site	2118	2129	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01930	.	conserved_region	2122	2125	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01930	.	terminator	3046	3086	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01930	.	stem_loop	3053	3072	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01930	.	stop_codon	3079	3079	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01930	.	polyA_site	3073	3086	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01930	.	engineered_region	3046	3086	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01930	.	CDS	984	1739	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01930	.	point_mutation	1475	1475	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01930	.	engineered_region	984	1739	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01930	.	engineered_region	984	985	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01930	.	CDS	984	1733	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01930	.	point_mutation	1157	1157	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01930	.	engineered_tag	1740	1764	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01930	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01930	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01930	.	terminator	1861	1901	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01930	.	stem_loop	1868	1887	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01930	.	stop_codon	1894	1894	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01930	.	polyA_site	1888	1901	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01930	.	engineered_region	1861	1901	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S01930
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtga
caaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaac
aataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataa
aattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattat
ttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggc
aatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattg
gaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcact
gggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactata
tagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaa
tatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagc
tcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactca
atacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaata
acactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttc
ccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatact
agatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaac
accaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggt
atgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactg
aagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcactt
gcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcg
cagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcg
taccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccgg
tcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattca
gaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgata
tagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac