##gff-version 3
##sequence-region BBa_S01897 1 2019
BBa_S01897	.	engineered_region	64	1032	.	+	0	ID=BBa_I0460;Name=BBa_I0460
BBa_S01897	.	terminator	904	983	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_S01897	.	stem_loop	915	958	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01897	.	engineered_region	904	983	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01897	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_S01897	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01897	.	CDS	82	870	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_S01897	.	CDS	82	831	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01897	.	tag	832	864	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01897	.	engineered_region	82	870	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01897	.	engineered_tag	871	895	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01897	.	start_codon	82	84	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01897	.	stop_codon	865	870	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01897	.	terminator	992	1032	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_S01897	.	stem_loop	999	1018	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01897	.	stop_codon	1025	1025	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01897	.	polyA_site	1019	1032	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01897	.	engineered_region	992	1032	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01897	.	engineered_region	1103	2019	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_S01897	.	ribosome_entry_site	1103	1114	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_S01897	.	conserved_region	1107	1110	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01897	.	terminator	1891	1970	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_S01897	.	stem_loop	1902	1945	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01897	.	engineered_region	1891	1970	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01897	.	engineered_region	1121	1882	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_S01897	.	sequence_alteration	1362	1362	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01897	.	engineered_region	1121	1882	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01897	.	tag	1838	1876	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01897	.	stop_codon	1877	1882	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01897	.	CDS	1121	1882	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01897	.	terminator	1979	2019	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_S01897	.	stem_loop	1986	2005	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01897	.	stop_codon	2012	2012	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01897	.	polyA_site	2006	2019	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01897	.	engineered_region	1979	2019	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01897	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S01897	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01897	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01897	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01897	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01897	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01897	.	promoter	1041	1094	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01897	.	engineered_region	1041	1094	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01897	.	promoter	1060	1065	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01897	.	non_covalent_binding_site	1041	1059	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01897	.	promoter	1083	1088	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01897	.	non_covalent_binding_site	1066	1084	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_S01897
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaac
accaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggt
atgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactg
aagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcactt
gcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcg
cagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcg
taccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccgg
tcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattca
gaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgata
tagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaag
aggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcga
cgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatc
tgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgct
accccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttctt
caaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaag
ggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatca
tggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgc
cgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtcc
gccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcg
gcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata