##gff-version 3
##sequence-region BBa_S01850 1 2164
BBa_S01850	.	CDS	227	1015	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S01850	.	tag	977	1009	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01850	.	engineered_region	227	1015	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01850	.	engineered_tag	1016	1040	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01850	.	start_codon	227	229	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01850	.	stop_codon	1010	1015	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01850	.	CDS	227	976	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01850	.	ribosome_entry_site	1248	1259	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01850	.	conserved_region	1252	1255	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01850	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01850	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01850	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01850	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01850	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01850	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01850	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01850	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01850	.	terminator	1049	1128	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01850	.	engineered_region	1049	1128	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01850	.	stem_loop	1060	1103	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01850	.	terminator	1137	1177	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01850	.	stem_loop	1144	1163	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01850	.	engineered_region	1137	1177	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01850	.	stop_codon	1170	1170	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01850	.	polyA_site	1164	1177	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01850	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01850	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01850	.	promoter	1186	1239	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01850	.	promoter	1205	1210	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01850	.	non_covalent_binding_site	1186	1204	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01850	.	promoter	1228	1233	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01850	.	engineered_region	1186	1239	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01850	.	non_covalent_binding_site	1211	1229	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01850	.	engineered_region	1266	2027	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01850	.	engineered_region	1266	2027	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01850	.	tag	1983	2021	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01850	.	stop_codon	2022	2027	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01850	.	CDS	1266	2027	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01850	.	sequence_alteration	1507	1507	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01850	.	terminator	2036	2115	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01850	.	engineered_region	2036	2115	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01850	.	stem_loop	2047	2090	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01850	.	terminator	2124	2164	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01850	.	stem_loop	2131	2150	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01850	.	engineered_region	2124	2164	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01850	.	stop_codon	2157	2157	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01850	.	polyA_site	2151	2164	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S01850
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacagtaaa
gaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaatta
ttagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtg
cagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaat
cgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcat
gcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaag
aatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttca
attattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattatta
acgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttat
cttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaag
gggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata