##gff-version 3
##sequence-region BBa_S01674 1 3387
BBa_S01674	.	ribosome_entry_site	2353	2364	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01674	.	conserved_region	2357	2360	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01674	.	promoter	912	965	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01674	.	engineered_region	912	965	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01674	.	promoter	931	936	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01674	.	non_covalent_binding_site	937	955	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01674	.	promoter	954	959	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01674	.	non_covalent_binding_site	912	930	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01674	.	CDS	2371	3120	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S01674	.	engineered_tag	3121	3145	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01674	.	CDS	2374	3081	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01674	.	tag	3082	3114	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01674	.	terminator	775	854	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01674	.	stem_loop	786	829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01674	.	engineered_region	775	854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01674	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_S01674	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S01674	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S01674	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S01674	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S01674	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S01674	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01674	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01674	.	CDS	992	2119	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S01674	.	engineered_tag	2120	2144	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01674	.	CDS	992	2119	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01674	.	engineered_region	992	2119	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01674	.	tag	2081	2119	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01674	.	terminator	2153	2232	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01674	.	stem_loop	2164	2207	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01674	.	engineered_region	2153	2232	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01674	.	terminator	3242	3282	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01674	.	stem_loop	3249	3268	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01674	.	polyA_site	3269	3282	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01674	.	stop_codon	3275	3275	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01674	.	engineered_region	3242	3282	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01674	.	promoter	2290	2343	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S01674	.	operator	2315	2331	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01674	.	promoter	2309	2314	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01674	.	promoter	2332	2337	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01674	.	operator	2292	2308	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01674	.	engineered_region	2290	2343	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01674	.	terminator	2241	2281	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01674	.	stem_loop	2248	2267	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01674	.	polyA_site	2268	2281	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01674	.	stop_codon	2274	2274	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01674	.	engineered_region	2241	2281	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01674	.	terminator	3154	3233	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01674	.	stem_loop	3165	3208	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01674	.	engineered_region	3154	3233	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01674	.	CDS	82	741	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01674	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01674	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01674	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01674	.	promoter	3291	3387	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S01674	.	promoter	3337	3342	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01674	.	non_covalent_binding_site	3347	3363	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01674	.	promoter	3384	3387	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01674	.	non_covalent_binding_site	3371	3387	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01674	.	promoter	3361	3366	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01674	.	engineered_region	3291	3387	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01674	.	non_covalent_binding_site	3296	3315	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01674	.	promoter	3348	3348	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01674	.	terminator	863	903	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01674	.	stem_loop	870	889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01674	.	polyA_site	890	903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01674	.	stop_codon	896	896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01674	.	engineered_region	863	903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01674	.	ribosome_entry_site	974	985	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01674	.	conserved_region	978	981	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S01674
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtc
gcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggg
aaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgtt
gctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgat
caactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttc
tcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcac
taatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacg
cgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcgg
cgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactg
gagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaac
gatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggat
acgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaac
cagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa
agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
aggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta
gtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaa
ttgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactag
atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaaga
aaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaa
tggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccct
tcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctg
ttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaag
cacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaag
ccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagac
ttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagta
cccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggc
gctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggttta
cgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata