##gff-version 3
##sequence-region BBa_Q09400 1 912
BBa_Q09400	.	promoter	859	912	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_Q09400	.	engineered_region	859	912	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_Q09400	.	non_covalent_binding_site	884	902	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_Q09400	.	non_covalent_binding_site	859	877	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_Q09400	.	promoter	901	906	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_Q09400	.	promoter	878	883	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_Q09400	.	terminator	810	850	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_Q09400	.	stop_codon	843	843	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_Q09400	.	polyA_site	837	850	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_Q09400	.	stem_loop	817	836	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_Q09400	.	engineered_region	810	850	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_Q09400	.	CDS	29	688	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_Q09400	.	engineered_tag	689	713	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_Q09400	.	CDS	32	648	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_Q09400	.	tag	649	682	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_Q09400	.	terminator	722	801	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_Q09400	.	engineered_region	722	801	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_Q09400	.	stem_loop	733	776	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_Q09400	.	ribosome_entry_site	1	22	.	+	0	ID=BBa_B0039;Name=BBa_B0039
>BBa_Q09400
tcacacaggaaaggcctcgatgtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgctt
aatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggc
atgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgcccttt
agaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatgga
gcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaac
aaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatca
agagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaactta
aatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatc
actactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagt
gatagagattgacatccctatcagtgatagagatactgagcac