##gff-version 3
##sequence-region BBa_Q07400 1 910
BBa_Q07400	.	promoter	857	910	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_Q07400	.	engineered_region	857	910	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_Q07400	.	non_covalent_binding_site	882	900	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_Q07400	.	non_covalent_binding_site	857	875	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_Q07400	.	promoter	899	904	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_Q07400	.	promoter	876	881	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_Q07400	.	CDS	27	686	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_Q07400	.	engineered_tag	687	711	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_Q07400	.	CDS	30	646	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_Q07400	.	tag	647	680	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_Q07400	.	ribosome_entry_site	1	20	.	+	0	ID=BBa_B0041;Name=BBa_B0041
BBa_Q07400	.	terminator	720	799	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_Q07400	.	engineered_region	720	799	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_Q07400	.	stem_loop	731	774	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_Q07400	.	terminator	808	848	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_Q07400	.	stop_codon	841	841	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_Q07400	.	polyA_site	835	848	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_Q07400	.	stem_loop	815	834	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_Q07400	.	engineered_region	808	848	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_Q07400
tcacacaggacggccggatgtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaa
tgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcat
gtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttag
aaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagc
aaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaa
ggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaag
agcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaatt
atttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaa
tgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtga
tagagattgacatccctatcagtgatagagatactgagcac