##gff-version 3
##sequence-region BBa_P20020 1 505
BBa_P20020	.	terminator	465	505	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_P20020	.	stop_codon	498	498	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_P20020	.	polyA_site	492	505	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_P20020	.	stem_loop	472	491	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_P20020	.	engineered_region	465	505	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_P20020	.	CDS	84	368	.	+	0	ID=BBa_C2002;Name=BBa_C2002
BBa_P20020	.	non_covalent_binding_site	174	233	.	+	0	ID=annotation1878171;Name=Zif268 finger 3;Parent=BBa_C2002
BBa_P20020	.	start_codon	84	86	.	+	0	ID=annotation1878168;Name=start codon;Parent=BBa_C2002
BBa_P20020	.	sequence_feature	234	248	.	+	0	ID=annotation1878173;Name=domain linker;Parent=BBa_C2002
BBa_P20020	.	non_covalent_binding_site	249	344	.	+	0	ID=annotation1878174;Name=GCN4 homodimerization domain;Parent=BBa_C2002
BBa_P20020	.	non_covalent_binding_site	126	146	.	+	0	ID=annotation1878170;Name=DNA recognition site;Parent=BBa_C2002
BBa_P20020	.	stop_codon	363	368	.	+	0	ID=annotation1878176;Name=double stop codon;Parent=BBa_C2002
BBa_P20020	.	tag	345	362	.	+	0	ID=annotation1878175;Name=hexaHis tag;Parent=BBa_C2002
BBa_P20020	.	non_covalent_binding_site	87	173	.	+	0	ID=annotation1878169;Name=Zif268 finger 2;Parent=BBa_C2002
BBa_P20020	.	non_covalent_binding_site	210	230	.	+	0	ID=annotation1878172;Name=DNA recognition site;Parent=BBa_C2002
BBa_P20020	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_P20020	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_P20020	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_P20020	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_P20020	.	terminator	377	456	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_P20020	.	stem_loop	388	431	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_P20020	.	engineered_region	377	456	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_P20020	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_P20020	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_P20020	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_P20020	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_P20020	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_P20020	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_P20020
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacc
cacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgta
aattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgc
tcgtctgaaaaaactggttggtgaacgtcatcaccatcaccatcactaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata