##gff-version 3
##sequence-region BBa_M30000 1 624
BBa_M30000	.	engineered_region	117	624	.	+	0	ID=BBa_I2012;Name=BBa_I2012
BBa_M30000	.	engineered_region	135	487	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_I2012
BBa_M30000	.	sequence_feature	333	482	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_M30000	.	sequence_alteration	221	221	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_M30000	.	sequence_alteration	212	212	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_M30000	.	CDS	135	482	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_M30000	.	promoter	160	169	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_M30000	.	sequence_feature	135	149	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_M30000	.	promoter	307	325	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_M30000	.	sequence_alteration	242	242	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_M30000	.	sequence_alteration	245	245	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_M30000	.	sequence_alteration	215	215	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_M30000	.	terminator	496	624	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I2012
BBa_M30000	.	terminator	584	624	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M30000	.	engineered_region	584	624	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M30000	.	polyA_site	611	624	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M30000	.	stop_codon	617	617	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M30000	.	stem_loop	591	610	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M30000	.	terminator	496	575	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M30000	.	engineered_region	496	575	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M30000	.	stem_loop	507	550	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M30000	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I2012
BBa_M30000	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M30000	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_M30000	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_M30000	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_M30000	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_M30000	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_M30000	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
>BBa_M30000
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgaccatgattacgccaagcgcg
caattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgg
gctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtga
gtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgca
gcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata