##gff-version 3 ##sequence-region BBa_M1721 1 1613 BBa_M1721 . engineered_region 1 1569 . + 0 ID=BBa_M1720;Name=BBa_M1720 BBa_M1721 . CDS 942 1145 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1721 . sequence_feature 942 1025 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 972 986 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1010 1025 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1094 1109 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 987 1001 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1127 1145 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1055 1070 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1036 1051 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1026 1109 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1071 1085 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1026 1035 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 951 965 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 942 950 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1110 1126 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1721 . CDS 1154 1357 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1721 . sequence_feature 1154 1237 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1184 1198 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1222 1237 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1306 1321 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1199 1213 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1339 1357 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1267 1282 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1248 1263 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1238 1321 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1283 1297 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1238 1247 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1163 1177 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1154 1162 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1322 1338 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1721 . CDS 730 933 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1721 . sequence_feature 730 813 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 760 774 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 798 813 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 882 897 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 775 789 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 915 933 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 843 858 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 824 839 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 814 897 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 859 873 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 814 823 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 739 753 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 730 738 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 898 914 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1721 . CDS 1366 1569 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1721 . sequence_feature 1366 1449 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1396 1410 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1434 1449 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1518 1533 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1411 1425 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1551 1569 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1479 1494 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1460 1475 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1450 1533 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1495 1509 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1450 1459 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1375 1389 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1366 1374 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 1534 1550 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1721 . CDS 518 721 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1721 . sequence_feature 518 601 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 548 562 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 586 601 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 670 685 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 563 577 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 703 721 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 631 646 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 612 627 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 602 685 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 647 661 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 602 611 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 527 541 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 518 526 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 686 702 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1721 . engineered_region 1 509 . + 0 ID=BBa_M1718;Name=BBa_M1718;Parent=BBa_M1720 BBa_M1721 . CDS 306 509 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1718 BBa_M1721 . sequence_feature 306 389 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 336 350 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 374 389 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 458 473 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 351 365 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 491 509 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 419 434 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 400 415 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 390 473 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1721 . sequence_feature 435 449 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 390 399 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 315 329 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1721 . sequence_feature 306 314 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1721 . sequence_feature 474 490 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1721 . CDS 1 297 . + 0 ID=BBa_M1716;Name=BBa_M1716;Parent=BBa_M1718 BBa_M1721 . tag 1578 1613 . + 0 ID=BBa_K844000;Name=BBa_K844000 BBa_M1721 . stop_codon 1608 1610 . + 0 ID=annotation2206608;Name=Stop;Parent=BBa_K844000 BBa_M1721 . sequence_feature 1578 1607 . + 0 ID=annotation2206607;Name=10x-Histidine Tag;Parent=BBa_K844000 BBa_M1721 . stop_codon 1611 1613 . + 0 ID=annotation2206609;Name=Stop;Parent=BBa_K844000 >BBa_M1721 atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgc gtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtct gatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccg atctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatgg tcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtgga tatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaag gtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggacca ggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaag gaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagc agcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggag gtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttc tggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggat atggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaagg tggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtgga caaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaagg accaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaa caaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcag cagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttct ggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctg gttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagcat catcaccatcaccaccatcatcaccattaataa