##gff-version 3
##sequence-region BBa_M1615 1 2179
BBa_M1615	.	ribosome_entry_site	1302	1316	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1615	.	ribosome_entry_site	1302	1316	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1615	.	ribosome_entry_site	1309	1313	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1615	.	engineered_region	1302	1316	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1615	.	promoter	1240	1293	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_M1615	.	engineered_region	1240	1293	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M1615	.	non_covalent_binding_site	1240	1258	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M1615	.	non_covalent_binding_site	1265	1283	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M1615	.	promoter	1259	1264	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M1615	.	promoter	1282	1287	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M1615	.	terminator	2051	2179	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1615	.	terminator	2139	2179	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1615	.	polyA_site	2166	2179	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1615	.	engineered_region	2139	2179	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1615	.	stem_loop	2146	2165	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1615	.	stop_codon	2172	2172	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1615	.	terminator	2051	2130	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1615	.	stem_loop	2062	2105	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1615	.	engineered_region	2051	2130	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1615	.	CDS	1323	2042	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_M1615	.	CDS	1323	2042	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_M1615	.	engineered_region	1	1231	.	+	0	ID=BBa_M1612;Name=BBa_M1612
BBa_M1615	.	terminator	1103	1231	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_M1612
BBa_M1615	.	terminator	1191	1231	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1615	.	polyA_site	1218	1231	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1615	.	engineered_region	1191	1231	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1615	.	stem_loop	1198	1217	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1615	.	stop_codon	1224	1224	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1615	.	terminator	1103	1182	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1615	.	stem_loop	1114	1157	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1615	.	engineered_region	1103	1182	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1615	.	engineered_region	453	1094	.	+	0	ID=BBa_I15040;Name=BBa_I15040;Parent=BBa_M1612
BBa_M1615	.	CDS	468	1094	.	+	0	ID=annotation1501146;Name=paskiba3_TetR;Parent=BBa_I15040
BBa_M1615	.	ribosome_entry_site	453	467	.	+	0	ID=annotation1501145;Name=paskiba3_rbs;Parent=BBa_I15040
BBa_M1615	.	promoter	1	444	.	+	0	ID=BBa_M1610;Name=BBa_M1610;Parent=BBa_M1612
BBa_M1615	.	engineered_region	1	444	.	+	0	ID=annotation2214866;Name=BBa_M1610;Parent=BBa_M1610
>BBa_M1615
ttatttgagtaaattaatccacgatcccagccattcttctgccggatcttccggaatgtcgtgatcaagaatgttgatc
ttcagtgtttcgcctgtctgttttgcaccggaatttttgagttctgcctcgagtttatcgatagccccacaaaaggtgt
catattcacgactgccaataccgattgcgccaaagcggaccgcagaaagatcgggcttctgttcctgcaatgcttcata
gaaaggagaaaggttgtccggaatatctccggcaccgtgggtggagctgataatcagccagatccccgaggcagataaa
tcttccaacagcggaccgtgcagcgtttcggtggtaaaacccgcctcttccagcttttcagccaggtgttctgctacat
attcggcaccgccgagggtgctgccgctgataagagtgatatctgccattactagagcattggtaggaattaatgatgt
ctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccg
taaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcc
ttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgta
ataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacaga
aaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgca
ctcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaa
cacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcctt
cttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaactt
ttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg
aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacact
tgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagt
gccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtca
agtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggaca
caaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttc
aaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggcc
ctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacat
ggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata