##gff-version 3
##sequence-region BBa_M1539 1 9254
BBa_M1539	.	tag	4569	4754	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1539	.	start_codon	4569	4571	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1539	.	tag	4569	4754	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1539	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1539	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1539	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1539	.	engineered_region	5509	9117	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1539	.	ribosome_entry_site	5509	5522	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1539	.	ribosome_entry_site	7661	7674	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1539	.	CDS	7681	9117	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1539	.	CDS	7681	9114	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1539	.	start_codon	7681	7683	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1539	.	stop_codon	9115	9117	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1539	.	CDS	5529	7652	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1539	.	stop_codon	7650	7652	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1539	.	start_codon	5529	5531	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1539	.	CDS	5529	7649	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1539	.	terminator	9126	9254	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1539	.	terminator	9214	9254	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1539	.	stem_loop	9221	9240	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1539	.	polyA_site	9241	9254	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1539	.	stop_codon	9247	9247	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1539	.	engineered_region	9214	9254	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1539	.	terminator	9126	9205	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1539	.	stem_loop	9137	9180	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1539	.	engineered_region	9126	9205	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1539	.	ribosome_entry_site	423	434	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1539	.	conserved_region	427	430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1539	.	ribosome_entry_site	4763	4774	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1539	.	conserved_region	4767	4770	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1539	.	ribosome_entry_site	4549	4560	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1539	.	conserved_region	4553	4556	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1539	.	engineered_region	932	4540	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1539	.	ribosome_entry_site	932	945	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1539	.	ribosome_entry_site	3084	3097	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1539	.	CDS	3104	4540	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1539	.	CDS	3104	4537	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1539	.	start_codon	3104	3106	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1539	.	stop_codon	4538	4540	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1539	.	CDS	952	3075	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1539	.	stop_codon	3073	3075	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1539	.	start_codon	952	954	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1539	.	CDS	952	3072	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1539	.	CDS	441	923	.	+	0	ID=BBa_M1538;Name=BBa_M1538
BBa_M1539	.	stop_codon	911	921	.	+	0	ID=annotation2172551;Name=stop;Parent=BBa_M1538
BBa_M1539	.	start_codon	441	461	.	+	0	ID=annotation2172550;Name=start;Parent=BBa_M1538
BBa_M1539	.	CDS	4781	5500	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1539	.	start_codon	4781	4783	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_M1539	.	CDS	4781	5500	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1539	.	tag	229	414	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1539	.	start_codon	229	231	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1539	.	tag	229	414	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
>BBa_M1539
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctat
ggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaag
aggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccct
gaccacatcagcataataatactagagaaagaggagaaatactagatggctttcaaaacgtgcggattcagcaagaaat
ggttggttattgccgttatagtcatgtgcctttgtactgaatgctattgtcactgtacgggtggagccgattgcacctc
ctgtactgatgcttgcacgggttgtggtaattgcccaaacgcgcatacctgtactgacagcaagaatgtttgtaaggcg
gcaacctgtacagatagtgagaattgtgtgaaagcccatacatgtaccggcagtacgaaatgtaacacggcgagaacat
gtactaactctaaggactgctttgaagcgaaaacgtgtaccgggagtacgcattgtcatcgtgcgaccacttgcactaa
ttcaaaagattgtttcgaagccaccacctgtacagggagttcgaattgttatacagcgaccacgtgtacagacagtacc
aattgctataaagctaccgcgtgtaccaattcgaccgggtgtccaggacattagtactagagattaaagaggagaatac
tagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtca
acccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtc
cctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgt
gaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgca
acccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgt
tgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaacc
ctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctgg
ttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccgg
tctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctg
gcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgca
actttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtacta
cagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctg
cgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaaca
ctatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggctt
caaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtgg
ctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattg
ttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgt
cctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatc
cgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggca
tcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggt
tctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataac
gtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctg
cgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctgg
cctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgag
gccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaa
tcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaaca
gggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactag
agattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatgg
agcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatc
tggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgt
aatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaa
atcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgc
tgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagac
ccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccag
aacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaatacc
aaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtc
tcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaa
caggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcc
tgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacgga
ctctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatct
ggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccgg
aagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcat
caaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaa
gatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattc
cactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgct
ggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggagaaatactagagttagcctatggaag
tcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaa
agaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgacca
catcagcataataatactagagaaagaggagaaatactagatggctagcaaaggagaagaacttttcactggagttgtc
ccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacat
acggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctc
ttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggt
tatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgata
cccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaa
ctataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaac
attgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccag
acaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtt
tgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagattaaagaggagaatactagat
ggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccg
gaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctgg
agctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaaga
tggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccg
cgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcag
gtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggt
tgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcac
cgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgc
gtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcact
gcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaacttt
ctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcc
cgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcg
ccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatc
aaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaag
tgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctggg
tgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgcc
ccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctga
actctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttt
tcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtg
ggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctga
ttgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgct
gctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaa
ctggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgt
ccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccac
ttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatc
atcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggta
aacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagatt
aaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcga
aacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaa
ctgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcc
tgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaa
accgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaa
ctgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgtt
accaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgt
aagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaag
gaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtg
ttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacagga
aaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtct
gcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactcta
tcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaa
agttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagat
gacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaag
tagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatca
gaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactg
tcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaag
aaagcgtcaccgaatctctgcacgaacgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata