##gff-version 3
##sequence-region BBa_M1519 1 7214
BBa_M1519	.	engineered_region	3496	7194	.	+	0	ID=BBa_M1006;Name=BBa_M1006
BBa_M1519	.	terminator	7156	7194	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_M1006
BBa_M1519	.	polyA_site	7187	7194	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_M1519	.	engineered_region	7156	7194	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_M1519	.	stem_loop	7165	7186	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_M1519	.	polyA_site	7156	7164	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_M1519	.	CDS	3559	5682	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1006
BBa_M1519	.	start_codon	3559	3561	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1519	.	stop_codon	5680	5682	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1519	.	CDS	3559	5679	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1519	.	engineered_region	3496	3552	.	+	0	ID=BBa_M1005;Name=BBa_M1005;Parent=BBa_M1006
BBa_M1519	.	promoter	3496	3530	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_M1005
BBa_M1519	.	ribosome_entry_site	3539	3552	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1005
BBa_M1519	.	CDS	5711	7147	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1006
BBa_M1519	.	CDS	5711	7144	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1519	.	start_codon	5711	5713	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1519	.	stop_codon	7145	7147	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1519	.	ribosome_entry_site	5691	5704	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1006
BBa_M1519	.	promoter	3074	3293	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1519	.	ribosome_entry_site	3282	3293	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1519	.	promoter	3074	3273	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1519	.	tag	3302	3487	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1519	.	start_codon	3302	3304	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1519	.	tag	3302	3487	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1519	.	ribosome_entry_site	7203	7214	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1519	.	conserved_region	7207	7210	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1519	.	CDS	1054	3065	.	+	0	ID=BBa_K654060;Name=BBa_K654060
BBa_M1519	.	stop_codon	3063	3065	.	+	0	ID=annotation2141921;Name=Stop;Parent=BBa_K654060
BBa_M1519	.	tag	3039	3062	.	+	0	ID=annotation2141922;Name=8-His Tag;Parent=BBa_K654060
BBa_M1519	.	start_codon	1054	1056	.	+	0	ID=annotation2141920;Name=Start;Parent=BBa_K654060
BBa_M1519	.	CDS	1054	3038	.	+	0	ID=annotation2141924;Name=Fatty Acyl-CoA Reductase;Parent=BBa_K654060
BBa_M1519	.	sequence_alteration	2685	2685	.	+	0	ID=annotation2141923;Name=A>C;Parent=BBa_K654060
BBa_M1519	.	CDS	1	1047	.	+	0	ID=BBa_K654062;Name=BBa_K654062
BBa_M1519	.	start_codon	1	3	.	+	0	ID=annotation2141929;Name=Start;Parent=BBa_K654062
BBa_M1519	.	stop_codon	1045	1047	.	+	0	ID=annotation2141930;Name=Stop;Parent=BBa_K654062
BBa_M1519	.	CDS	1	1020	.	+	0	ID=annotation2141940;Name=Fatty Acyl ACP Reductase;Parent=BBa_K654062
BBa_M1519	.	tag	1021	1044	.	+	0	ID=annotation2141941;Name=8-His Tag;Parent=BBa_K654062
>BBa_M1519
atgtttggtcttattggtcatctcacgagtttagaacacgcccaagcggttgctgaagatttaggctatcctgagtacg
ccaaccaaggcctggatttttggtgttcggctcctccccaagtggttgataattttcaggtgaaaagtgtgacggggca
ggtgattgaaggcaaatatgtggagtcttgctttttgccggaaatgttaacccaacggcggatcaaagcggccattcgt
aaaatcctcaatgctatggccctggcccaaaaggtgggcttggatattacggccctgggaggcttttcttcaatcgtat
ttgaagaatttaacctcaagcaaaataatcaagtccgcaatgtggaactagattttcagcggttcaccactggtaatac
ccacaccgcttatgtgatctgccgtcaggtcgagtctggagctaaacagttgggtattgatctaagtcaggcaacggta
gcggtttgtggcgccacgggagatattggtagcgccgtatgtcgttggttagatagcaaacatcaagttaaggaattat
tgctaattgcccgtaaccgccaaagattggaaaatctccaagaggaattgggtcggggcaaaattatggatttggaaac
agccctgccccaggcagatattattgtttgggtggctagtatgcccaagggggtagaaattgcgggggaaatgctgaaa
aagccctgtttgattgtggatgggggctatcccaagaatttagacaccagggtgaaagcggatggggtgcatattctca
agggggggattgtagaacattcccttgatattacctgggaaattatgaagattgtggagatggatattccctcccggca
aatgttcgcctgttttgcggaggccattttgctagagtttgagggctggcgcactaatttttcctggggccgcaaccaa
atttccgttaataaaatggaggcgattggtgaagcttctgtcaagcatggcttttgccctttagtagctcttcatcatc
accatcatcaccatcactagtactagatgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttga
gaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggag
cgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaa
cgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagc
ccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcat
gtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatc
atccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccc
tggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcag
aagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatt
tcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatcc
gtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccgg
atgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgcattactggtgcgcttc
tggatgacatgggcattccgccctcggtgatgtccttcattaattacccgacccgttttgatacccgggagctggagcg
ggttctgaagggcacagacattgaggtgccgcgtctgccgtcctatgccccggttatctgggactactgggagcgcaat
ctggacccggacctgttcaaggaccgcaccctcaagggcacggttgaaggtaaggtttgcgtggtcaccggcgcgacct
cgggtattggcctggcaacggcagagaagctggcagaggccggtgccattctggtcattggtgcgcgcaccaaggaaac
tctggatgaagtggcggccagtctggaggccaagggtggcaacgtgcatgcgtaccagtgcgacttttcggacatggac
gactgcgaccgctttgtgaagacggtgctggataatcacggccacgtggatgtactggtgaataacgcgggtcgctcca
tccgccgctcgctggcgttgtcttttgaccggttccacgattttgagcggaccatgcagctgaactactttggctccgt
tcggctgatcatgggctttgcgccagccatgctggagcgtcgccgcgggcacgtggtgaatatttcttccatcggggta
cttaccaacgctccgcgtttctcggcctatgtctcctcgaaatccgcactggacgcgttcagccgctgtgccgctgccg
aatggtcggatcgcaacgtgaccttcaccaccatcaacatgccgttggtgaaaacgccgatgatcgcgcccaccaagat
ctacgattccgtgccgacgctgacgccggatgaagccgcccagatggtggcggatgcgattgtgtaccggcccaagcgc
attgccacccgtcttggcgtgttcgcgcaggttctgcatgcgctggcaccgaagatgggtgagatcattatgaacactg
gctaccggatgttcccggattctccagcagccgctggcagcaagtccggcgaaaagccgaaagtctctaccgagcaggt
ggcctttgcggcgattatgcgggggatatactggagcatcatcaccatcatcaccatcactaatactagagcaatacgc
aaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtg
agcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtt
gtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctatggaagtca
gggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaaga
accgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacat
cagcataataatactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatac
tagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtca
acccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtc
cctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgt
gaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgca
acccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgt
tgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaacc
ctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctgg
ttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgtggttgttgtgttcgaaatcattctgtccgg
tctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctg
gcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgca
actttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtacta
cagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctg
cgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaaca
ctatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggctt
caaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtgg
ctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattg
ttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgt
cctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatc
cgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggca
tcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggt
tctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataac
gtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctg
cgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctgg
cctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgag
gccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaa
tcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaaca
gggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactag
agattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatgg
agcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatc
tggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgt
aatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaa
atcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgc
tgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagac
ccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccag
aacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaatacc
aaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtc
tcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaa
caggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcc
tgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacgga
ctctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatct
ggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccgg
aagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcat
caaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaa
gatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattc
cactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgct
ggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaaaaaaaaccccgcccctgacagggcggggttt
ttttttactagagaaagaggagaaa