LOCUS BBa_K892003 4118 bp DNA linear 12-Dec-2025 DEFINITION OmpR promoter + LacI inverter driving red luciferase ACCESSION BBa_K892003 VERSION BBa_K892003.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 misc_feature 1224..1262 /label=LVA CDS 135..1262 /label=lacI-LVA misc_feature 135..1262 /label=BBa_C0012 misc_feature 1263..1287 /label=Help:Barcodes CDS 135..1262 /label=BBa_C0012 stem_loop 1307..1350 /label=stem_loop misc_feature 1296..1375 /label=BBa_B0010 terminator 1296..1375 /label=BBa_B0010 stem_loop 1391..1410 /label=T7 TE misc_feature 1417..1417 /label=stop polyA_site 1411..1424 /label=polya misc_feature 1384..1424 /label=BBa_B0012 terminator 1384..1424 /label=BBa_B0012 terminator 1296..1424 /label=BBa_B0015 misc_feature 1435..1451 /label=lac O1 promoter 1452..1457 /label=-35 misc_feature 1458..1474 /label=lac O1 promoter 1475..1480 /label=-10 misc_feature 1433..1486 /label=BBa_R0011 promoter 1433..1486 /label=BBa_R0011 misc_feature 117..1487 /label=BBa_Q04121 RBS 1496..1512 /label=rbs CDS 1513..2439 /label=Luciferin regenerating enzyme (L. cruciata) misc_feature 2440..2445 /label=double stop codon misc_feature 2446..2451 /label=BamH1 site RBS 2452..2468 /label=rbs CDS 2469..4112 /label=Red luciferase (286) (L. cruciata) misc_feature 4113..4118 /label=double stop codon misc_feature 3325..3325 /label=G to A: Ser286Asn misc_feature 1496..4118 /label=BBa_K325210 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 181 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 241 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 301 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 361 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 421 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 481 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 541 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 601 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 661 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 721 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 781 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 841 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 901 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 961 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 1021 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 1081 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 1141 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 1201 tttcccgact ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat 1261 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1321 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1381 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agaattgtga 1441 gcggataaca attgacattg tgagcggata acaagatact gagcacatac tagagttaag 1501 gaggtaaaaa aaatggcccc gaccgtggaa caaattgtgg agctgggtac gtatctgctg 1561 gccgaaagcc cgcactggga cgatgaaacg caaagcctgt atttcgtcga tatcgttggt 1621 cgcagcgtga acaaatacgt tccgacgacc aagacgcata cccaactgaa attcgacaag 1681 aatccgagct tcatcatccc ggtgaagggt tgttccgacc gttttatcgt cagcctggaa 1741 cgcgagatca acctgctgac ctgggacggc gcatcctctg caccatccaa gattgagaag 1801 atcgcggttt tcgataatac gccagagaag agcgaaaacc gtctgaacga tggtaaggca 1861 gatccgctgg gcaacctgtg ggcgggtacc atgaatatgg gttctgacca taccaccggc 1921 acgccggtgc gcggtaccct gagcagcctg agcaacaagc aagtcaaaga gcacgtcagc 1981 gaggtctgca tcagcaatgg cctggcatgg agcaaggacc tgaagaaatt ctattacatt 2041 gatagcgctg ttcgtcaagt tgaccagttc gatttcgatg cgaaaaacct gagcttgagc 2101 aatcgtcaac cgctgttcac ctttgacaaa cacggcatca tgggtagccc ggatggtcag 2161 accatcgatg cggagggtaa cctgtgggtt gccacctgtc agggcgataa agtcctgaag 2221 attgatactt ctaccccgga aaccctgctg ggtattgtgg agattccgga acatcaggtg 2281 acgagcgttt gcatcggcgg tgcagaactg aacgttttgt atgtgacgac cgcgtccatt 2341 aaactgcctg gtgcggatga aaccaagccg atgaaaggcg ccatctataa agtcaccggt 2401 ctgggtgtca aaggtctgcc gggtgaccgt gttaagctgt aataaggatc cttaaggagg 2461 taaaaaaaat ggagaacatg gagaacgacg agaatattgt ggttggtccg aaaccgttct 2521 acccgatcga agagggcagc gcgggtaccc agctgcgtaa atacatggaa cgctacgcga 2581 aactgggtgc gatcgcgttc acgaatgcgg ttaccggcgt ggactattcc tatgcggagt 2641 atttggaaaa gtcgtgttgt ctgggcaagg cgttacagaa ttatggcctg gtggtcgatg 2701 gccgtatcgc gctgtgcagc gagaactgtg aagagttttt cattccggtg atcgcgggtt 2761 tgtttattgg cgtgggcgtt gcaccgacca atgaaatcta cacgctgcgc gaactggtgc 2821 actccctggg tatctctaag ccgacgatcg ttttcagctc caagaaaggt ttggataaag 2881 ttattacggt gcaaaagacg gtcaccacga tcaaaacgat tgtgatcctg gacagcaaag 2941 ttgattaccg cggttatcaa tgcctggata cgttcatcaa gcgtaacacc ccgccaggtt 3001 ttcaagcatc tagcttcaaa acggtggagg ttgaccgtaa agaacaagtc gcactgatta 3061 tgaatagctc gggtagcacg ggcctgccaa agggtgtgca gctgacccat gaaaacaccg 3121 ttacccgttt tagccacgcg cgtgatccga tttacggcaa tcaagtgtcg ccgggtacgg 3181 ctgttctgac tgttgtccct tttcaccatg gttttggcat gtttaccact ctgggttacc 3241 tgatttgcgg ttttcgcgtc gttatgctga ccaagtttga tgaagaaacc ttcctgaaaa 3301 ccctgcaaga ctacaagtgc actaatgtga ttctggtccc gacgctgttc gcgatcctga 3361 acaaaagcga gctgctgaat aagtacgacc tgagcaattt ggtggagatt gcgagcggcg 3421 gtgctccgtt gagcaaagaa gtaggcgagg cagtggcacg tcgctttaac ctgcctggcg 3481 tccgtcaagg ttacggtttg actgaaacca cgagcgcgat tatcatcacc ccggaaggtg 3541 acgataaacc tggcgctagc ggtaaggtcg tgccgttgtt caaggctaaa gttattgatc 3601 tggacaccaa gaagagcctg ggcccgaatc gccgtggtga agtttgcgtt aagggcccga 3661 tgctgatgaa aggttatgta aacaatccgg aagcaacgaa agagctgatc gacgaagagg 3721 gctggctgca cactggcgat atcggctact acgatgaaga gaaacacttt ttcattgttg 3781 atcgcctgaa aagcctgatc aagtacaagg gttaccaagt cccgccagcg gagctggaaa 3841 gcgtactgtt gcagcacccg tcgattttcg acgccggtgt ggctggtgtg cctgatccgg 3901 tggctggcga actgccgggt gctgtcgtgg tgctggagag cggtaagaat atgaccgaaa 3961 aagaagtcat ggactatgtg gcgagccaag tgtccaatgc aaaacgtctg cgtggcggtg 4021 ttcgctttgt ggacgaagtg ccgaaaggtc tgaccggcaa aattgacggt cgtgcaattc 4081 gtgagattct gaagaaaccg gtcgcgaaaa tgtaataa //