LOCUS       BBa_K861169             1961 bp    DNA     linear       09-Dec-2025
DEFINITION  Indirect regulatory device, activated at high glucose concentration
ACCESSION   BBa_K861169
VERSION     BBa_K861169.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     misc_feature    144..147
                     /label=conserved
     RBS             140..151
                     /label=BBa_B0034
     CDS             161..868
                     /label=cI lambda
     misc_feature    869..901
                     /label=LVA
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             158..932
                     /label=BBa_C0051
     terminator      941..1035
                     /label=BBa_B0024
     promoter        1081..1086
                     /label=-10
     promoter        1058..1063
                     /label=-35
     misc_feature    1068..1084
                     /label=OR1
     misc_feature    1044..1060
                     /label=OR2
     misc_feature    1044..1092
                     /label=BBa_R0051
     promoter        1044..1092
                     /label=BBa_R0051
     misc_feature    1105..1108
                     /label=conserved
     RBS             1101..1112
                     /label=BBa_B0034
     CDS             1119..1793
                     /label=mrfp1
     misc_feature    1800..1824
                     /label=Help:Barcodes
     CDS             1119..1799
                     /label=BBa_E1010
     stem_loop       1844..1887
                     /label=stem_loop
     misc_feature    1833..1912
                     /label=BBa_B0010
     terminator      1833..1912
                     /label=BBa_B0010
     stem_loop       1928..1947
                     /label=T7 TE
     misc_feature    1954..1954
                     /label=stop
     polyA_site      1948..1961
                     /label=polya
     misc_feature    1921..1961
                     /label=BBa_B0012
     terminator      1921..1961
                     /label=BBa_B0012
     misc_feature    1101..1961
                     /label=BBa_I13507
     misc_feature    1044..1961
                     /label=BBa_I763007
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt
      181 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa
      241 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt
      301 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa
      361 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat
      421 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc
      481 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga
      541 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg
      601 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct
      661 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga
      721 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa
      781 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc
      841 tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag aaataataaa aaagccggat
      961 taataatctg gctttttata ttctctctct agtatataaa cgcagaaagg cccacccgaa
     1021 ggtgagccag tgtgatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt
     1081 gataatggtt gctactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
     1141 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     1201 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
     1261 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     1321 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     1381 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
     1441 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     1501 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     1561 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     1621 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     1681 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
     1741 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
     1801 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1861 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1921 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//