##gff-version 3
##sequence-region BBa_K819004 1 2783
BBa_K819004	.	promoter	1	152	.	+	0	ID=BBa_K819003;Name=BBa_K819003
BBa_K819004	.	promoter	1	152	.	+	0	ID=annotation2181611;Name=ColE promoter;Parent=BBa_K819003
BBa_K819004	.	engineered_region	161	2783	.	+	0	ID=BBa_K325210;Name=BBa_K325210
BBa_K819004	.	CDS	1134	2777	.	+	0	ID=annotation2080154;Name=Red luciferase (286) (L. cruciata);Parent=BBa_K325210
BBa_K819004	.	stop_codon	1105	1110	.	+	0	ID=annotation2080151;Name=double stop codon;Parent=BBa_K325210
BBa_K819004	.	CDS	178	1104	.	+	0	ID=annotation2080150;Name=Luciferin regenerating enzyme (L. cruciata);Parent=BBa_K325210
BBa_K819004	.	ribosome_entry_site	161	177	.	+	0	ID=annotation2080149;Name=rbs;Parent=BBa_K325210
BBa_K819004	.	sequence_alteration	1990	1990	.	+	0	ID=annotation2103099;Name=G to A: Ser286Asn;Parent=BBa_K325210
BBa_K819004	.	ribosome_entry_site	1117	1133	.	+	0	ID=annotation2080153;Name=rbs;Parent=BBa_K325210
BBa_K819004	.	non_covalent_binding_site	1111	1116	.	+	0	ID=annotation2080152;Name=BamH1 site;Parent=BBa_K325210
BBa_K819004	.	stop_codon	2778	2783	.	+	0	ID=annotation2080155;Name=double stop codon;Parent=BBa_K325210
>BBa_K819004
tgtttttttgatcgttttcacaaaaatggaagtccacagtcttgacagggaaaatgcagcggcgtagcttttatgctgt
atataaaaccagtggttatatgtacagtatttatttttaacttattgttttaaaagtcaaagaggattttatatactag
agttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccg
cactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgacca
agacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgt
cagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggtt
ttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggta
ccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaa
agagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagc
gctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttg
acaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcaggg
cgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacg
agcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaa
ccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgta
ataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttct
acccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgtt
cacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacag
aattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgg
gtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctc
taagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaa
acgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgc
caggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcggg
tagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatt
tacggcaatcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttaccactc
tgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaaga
ctacaagtgcactaatgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgac
ctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgcttta
acctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataa
acctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccg
aatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagc
tgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcg
cctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcg
attttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcg
gtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgt
tcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccg
gtcgcgaaaatgtaataa