##gff-version 3
##sequence-region BBa_K750115 1 3938
BBa_K750115	.	engineered_region	1	1068	.	+	0	ID=BBa_K750104;Name=BBa_K750104
BBa_K750115	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750104
BBa_K750115	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750115	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750115	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750115	.	terminator	940	1068	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750104
BBa_K750115	.	terminator	1028	1068	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750115	.	stem_loop	1035	1054	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750115	.	polyA_site	1055	1068	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750115	.	stop_codon	1061	1061	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750115	.	engineered_region	1028	1068	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750115	.	terminator	940	1019	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750115	.	stem_loop	951	994	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750115	.	engineered_region	940	1019	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750115	.	CDS	157	931	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K750104
BBa_K750115	.	engineered_tag	907	931	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K750115	.	CDS	160	867	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K750115	.	tag	868	900	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K750115	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750104
BBa_K750115	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750115	.	engineered_region	1077	3938	.	+	0	ID=BBa_K750114;Name=BBa_K750114
BBa_K750115	.	engineered_region	1077	2959	.	+	0	ID=BBa_K750102;Name=BBa_K750102;Parent=BBa_K750114
BBa_K750115	.	CDS	2048	2822	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K750102
BBa_K750115	.	engineered_tag	2798	2822	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K750115	.	CDS	2051	2758	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K750115	.	tag	2759	2791	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K750115	.	engineered_region	1120	2021	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K750102
BBa_K750115	.	terminator	1919	1959	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K750115	.	stem_loop	1926	1945	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750115	.	polyA_site	1946	1959	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750115	.	stop_codon	1952	1952	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750115	.	engineered_region	1919	1959	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750115	.	terminator	1831	1910	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K750115	.	stem_loop	1842	1885	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750115	.	engineered_region	1831	1910	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750115	.	CDS	1138	1797	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K750115	.	engineered_tag	1798	1822	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K750115	.	CDS	1141	1757	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K750115	.	tag	1758	1791	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K750115	.	promoter	1968	2021	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K750115	.	engineered_region	1968	2021	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K750115	.	promoter	1987	1992	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K750115	.	non_covalent_binding_site	1993	2011	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K750115	.	promoter	2010	2015	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K750115	.	non_covalent_binding_site	1968	1986	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K750115	.	ribosome_entry_site	1120	1131	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K750115	.	conserved_region	1124	1127	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750115	.	terminator	2831	2959	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750102
BBa_K750115	.	terminator	2919	2959	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750115	.	stem_loop	2926	2945	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750115	.	polyA_site	2946	2959	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750115	.	stop_codon	2952	2952	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750115	.	engineered_region	2919	2959	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750115	.	terminator	2831	2910	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750115	.	stem_loop	2842	2885	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750115	.	engineered_region	2831	2910	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750115	.	ribosome_entry_site	2030	2041	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750102
BBa_K750115	.	conserved_region	2034	2037	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750115	.	promoter	1077	1111	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K750102
BBa_K750115	.	engineered_region	2968	3938	.	+	0	ID=BBa_K750103;Name=BBa_K750103;Parent=BBa_K750114
BBa_K750115	.	engineered_region	3043	3801	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K750103
BBa_K750115	.	tag	3759	3795	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K750115	.	CDS	3046	3758	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K750115	.	start_codon	3043	3045	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K750115	.	stop_codon	3799	3801	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K750115	.	stop_codon	3796	3798	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K750115	.	promoter	2968	3016	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K750103
BBa_K750115	.	engineered_region	2968	3016	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K750115	.	non_covalent_binding_site	2992	3008	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K750115	.	promoter	3005	3010	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K750115	.	non_covalent_binding_site	2968	2984	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K750115	.	promoter	2982	2987	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K750115	.	terminator	3810	3938	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750103
BBa_K750115	.	terminator	3898	3938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750115	.	stem_loop	3905	3924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750115	.	polyA_site	3925	3938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750115	.	stop_codon	3931	3931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750115	.	engineered_region	3898	3938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750115	.	terminator	3810	3889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750115	.	stem_loop	3821	3864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750115	.	engineered_region	3810	3889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750115	.	ribosome_entry_site	3025	3036	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750103
BBa_K750115	.	conserved_region	3029	3032	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K750115
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaa
aatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatg
gcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttc
aatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgtt
ttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagca
caaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcc
aagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagactt
gggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacc
caatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgc
tgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatg
ctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctg
cttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtatt
ggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccc
tttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgat
ggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgcc
aacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaaga
tcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatc
gaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaac
ttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtag
atcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatc
agtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagca
caaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatga
acttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatc
aatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcg
ccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttc
tcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaacc
aaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagct
ttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttggggg
tgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatg
atcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaa
acgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcg
gtgataatggttgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagta
gcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata