##gff-version 3
##sequence-region BBa_K750114 1 2862
BBa_K750114	.	engineered_region	1892	2862	.	+	0	ID=BBa_K750103;Name=BBa_K750103
BBa_K750114	.	promoter	1892	1940	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K750103
BBa_K750114	.	promoter	1906	1911	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K750114	.	promoter	1929	1934	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K750114	.	non_covalent_binding_site	1916	1932	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K750114	.	non_covalent_binding_site	1892	1908	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K750114	.	engineered_region	1892	1940	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K750114	.	terminator	2734	2862	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750103
BBa_K750114	.	terminator	2734	2813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750114	.	stem_loop	2745	2788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750114	.	engineered_region	2734	2813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750114	.	terminator	2822	2862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750114	.	stop_codon	2855	2855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750114	.	engineered_region	2822	2862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750114	.	stem_loop	2829	2848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750114	.	polyA_site	2849	2862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750114	.	engineered_region	1967	2725	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K750103
BBa_K750114	.	start_codon	1967	1969	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K750114	.	stop_codon	2723	2725	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K750114	.	CDS	1970	2682	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K750114	.	tag	2683	2719	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K750114	.	stop_codon	2720	2722	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K750114	.	ribosome_entry_site	1949	1960	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750103
BBa_K750114	.	conserved_region	1953	1956	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750114	.	engineered_region	1	1883	.	+	0	ID=BBa_K750102;Name=BBa_K750102
BBa_K750114	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750102
BBa_K750114	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750114	.	CDS	972	1746	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K750102
BBa_K750114	.	tag	1683	1715	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K750114	.	engineered_tag	1722	1746	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K750114	.	CDS	975	1682	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K750114	.	terminator	1755	1883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750102
BBa_K750114	.	terminator	1755	1834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750114	.	stem_loop	1766	1809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750114	.	engineered_region	1755	1834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750114	.	terminator	1843	1883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750114	.	stop_codon	1876	1876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750114	.	engineered_region	1843	1883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750114	.	stem_loop	1850	1869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750114	.	polyA_site	1870	1883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750114	.	engineered_region	44	945	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K750102
BBa_K750114	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K750114	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750114	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750114	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K750114	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750114	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K750114	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750114	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750114	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750114	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750114	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K750114	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K750114	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K750114	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K750114	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K750114	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K750114	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K750114	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K750114	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K750114	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K750114	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K750102
>BBa_K750114
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgcct
taaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcag
tcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaag
ttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcact
tagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaa
ggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattcca
tgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagcc
aggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgttt
ttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtc
agtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtg
tagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaaca
ccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgcgtaaa
ggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtca
gtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgac
tttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacac
gtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaa
cattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatc
aaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaa
ttggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaa
gagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcct
gctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata