##gff-version 3
##sequence-region BBa_K750112 1 2846
BBa_K750112	.	engineered_region	980	2846	.	+	0	ID=BBa_K750111;Name=BBa_K750111
BBa_K750112	.	ribosome_entry_site	1023	1034	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750111
BBa_K750112	.	conserved_region	1027	1030	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750112	.	engineered_region	1951	2709	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K750111
BBa_K750112	.	CDS	1954	2666	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K750112	.	stop_codon	2707	2709	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K750112	.	stop_codon	2704	2706	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K750112	.	start_codon	1951	1953	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K750112	.	tag	2667	2703	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K750112	.	terminator	2718	2846	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750111
BBa_K750112	.	terminator	2806	2846	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750112	.	engineered_region	2806	2846	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750112	.	stem_loop	2813	2832	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750112	.	stop_codon	2839	2839	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750112	.	polyA_site	2833	2846	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750112	.	terminator	2718	2797	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750112	.	engineered_region	2718	2797	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750112	.	stem_loop	2729	2772	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750112	.	CDS	1041	1725	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K750111
BBa_K750112	.	engineered_tag	1701	1725	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K750112	.	CDS	1044	1660	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K750112	.	tag	1661	1694	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K750112	.	promoter	1871	1924	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K750111
BBa_K750112	.	promoter	1913	1918	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K750112	.	promoter	1890	1895	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K750112	.	engineered_region	1871	1924	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K750112	.	non_covalent_binding_site	1896	1914	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K750112	.	non_covalent_binding_site	1871	1889	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K750112	.	ribosome_entry_site	1933	1944	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750111
BBa_K750112	.	conserved_region	1937	1940	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750112	.	terminator	1734	1862	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750111
BBa_K750112	.	terminator	1822	1862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750112	.	engineered_region	1822	1862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750112	.	stem_loop	1829	1848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750112	.	stop_codon	1855	1855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750112	.	polyA_site	1849	1862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750112	.	terminator	1734	1813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750112	.	engineered_region	1734	1813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750112	.	stem_loop	1745	1788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750112	.	promoter	980	1014	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K750111
BBa_K750112	.	CDS	1041	1700	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K750112	.	engineered_tag	1701	1725	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K750112	.	CDS	1044	1660	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K750112	.	tag	1661	1694	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K750112	.	engineered_region	1	971	.	+	0	ID=BBa_K750103;Name=BBa_K750103
BBa_K750112	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750103
BBa_K750112	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750112	.	terminator	843	971	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750103
BBa_K750112	.	terminator	931	971	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750112	.	engineered_region	931	971	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750112	.	stem_loop	938	957	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750112	.	stop_codon	964	964	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750112	.	polyA_site	958	971	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750112	.	terminator	843	922	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750112	.	engineered_region	843	922	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750112	.	stem_loop	854	897	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750112	.	engineered_region	76	834	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K750103
BBa_K750112	.	CDS	79	791	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K750112	.	stop_codon	832	834	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K750112	.	stop_codon	829	831	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K750112	.	start_codon	76	78	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K750112	.	tag	792	828	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K750112	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K750103
BBa_K750112	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K750112	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K750112	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K750112	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K750112	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
>BBa_K750112
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgc
gtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaa
gacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagat
ggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatg
gaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaac
gaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaa
ggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaaga
ggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatc
gaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagc
gggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctg
gcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacattta
ggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactag
agaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgc
taaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaa
ggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggt
ccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacat
ccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttca
ctggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaagg
tgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtc
actactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgcca
tgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtt
tgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaa
ttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaa
ttagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgt
ccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtc
cttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta