##gff-version 3
##sequence-region BBa_K741021 1 1574
BBa_K741021	.	engineered_region	1	1138	.	+	0	ID=BBa_K741001;Name=BBa_K741001
BBa_K741021	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K741001
BBa_K741021	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K741021	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K741021	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K741021	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K741021	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K741021	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K741021	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K741021	.	engineered_region	209	1138	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_K741001
BBa_K741021	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K741021	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K741021	.	terminator	1098	1138	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K741021	.	engineered_region	1098	1138	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K741021	.	stop_codon	1131	1131	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K741021	.	polyA_site	1125	1138	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K741021	.	stem_loop	1105	1124	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K741021	.	terminator	1010	1089	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K741021	.	stem_loop	1021	1064	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K741021	.	engineered_region	1010	1089	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K741021	.	CDS	227	976	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K741021	.	tag	938	970	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K741021	.	engineered_tag	977	1001	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K741021	.	CDS	230	937	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K741021	.	engineered_region	1147	1574	.	+	0	ID=BBa_K741016;Name=BBa_K741016
BBa_K741021	.	sequence_feature	1237	1437	.	+	0	ID=BBa_K741000;Name=BBa_K741000;Parent=BBa_K741016
BBa_K741021	.	terminator	1446	1574	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K741016
BBa_K741021	.	terminator	1446	1525	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K741021	.	stem_loop	1457	1500	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K741021	.	engineered_region	1446	1525	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K741021	.	terminator	1534	1574	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K741021	.	engineered_region	1534	1574	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K741021	.	stop_codon	1567	1567	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K741021	.	polyA_site	1561	1574	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K741021	.	stem_loop	1541	1560	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K741021	.	promoter	1147	1228	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_K741016
BBa_K741021	.	promoter	1217	1222	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_K741021	.	non_covalent_binding_site	1179	1195	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_K741021	.	non_covalent_binding_site	1155	1171	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_K741021	.	sequence_alteration	1205	1211	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_K741021	.	promoter	1194	1199	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
>BBa_K741021
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcacaaa
aaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaactt
ggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatg
cattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccag
agaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcat
gttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaa
aagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcc
tgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgat
gagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcc
catgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacga
cgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacg
cacggtgttagatatttataaatagtggtgatagatttaacgttactagagttatgctgttgtttttttgttactcggg
aagggctttacctcttccgcataaacgcttccatcagcgtttatagttaaaaaaatctttcggcctgcatgaatggcct
tgttgatcgcgctttgatatacgccgagatctttagctgtcttggtttgcccaaagcgcattgcataatctttcagggt
tatgcgttgttccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata