BBa_K729015

BBa_K729015 Version 1

Component

This part has been discontinued.

Please consider using BBa_K118011 as a replacement.

Source: http://parts.igem.org/Part:BBa_K729015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erick Miguel Ramos-Martinez

Date created: 2012-09-12 11:00:00

Date modified: 2015-05-08 01:13:06

pcstA+RBS+T7RNAP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K729015_sequence (Version 1)

Description

This divice contains a glucose-repressible promoter (Part:BBa_K118011) ligated with a GFP generator (Part:BBa_E0840). Low glucose concentration promotes the transcription of the GFP as the complex between cAMP and CRP binds the cstA promoter.

Notes

-

Source

E.coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cAMP receptor protein binding site	1,42	non_covalent_binding_site, feature/binding
RNA polymerase binding site	101,131	non_covalent_binding_site, feature/binding
BBa_K118011	1,131	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K118011/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K729015/1

Member of these Collections

• iGEM Parts Registry