##gff-version 3
##sequence-region BBa_K658002 1 5291
BBa_K658002	.	ribosome_entry_site	4654	4668	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K658002	.	ribosome_entry_site	4661	4665	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K658002	.	engineered_region	4654	4668	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K658002	.	ribosome_entry_site	4654	4668	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K658002	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K658002	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K658002	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K658002	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K658002	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K658002	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K658002	.	CDS	4675	5154	.	+	0	ID=BBa_K176003;Name=BBa_K176003
BBa_K658002	.	stop_codon	5149	5154	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K658002	.	sequence_alteration	4846	4847	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K658002	.	engineered_region	4675	4839	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K658002	.	primer_binding_site	4862	4889	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K658002	.	point_mutation	4839	4839	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K658002	.	point_mutation	4929	4929	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K658002	.	start_codon	4675	4677	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K658002	.	primer_binding_site	4715	4738	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K658002	.	sequence_alteration	4690	4690	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K658002	.	engineered_region	4846	5151	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K658002	.	primer_binding_site	4695	4711	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K658002	.	primer_binding_site	4715	4731	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K658002	.	CDS	4675	5148	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K658002	.	engineered_region	870	4645	.	+	0	ID=BBa_K568000;Name=BBa_K568000
BBa_K658002	.	engineered_region	870	2268	.	+	0	ID=BBa_K322123;Name=BBa_K322123;Parent=BBa_K568000
BBa_K658002	.	ribosome_entry_site	1499	1513	.	+	0	ID=annotation2083046;Name=rbs;Parent=BBa_K322123
BBa_K658002	.	CDS	932	1498	.	+	0	ID=annotation2083045;Name=Heme oxygenase;Parent=BBa_K322123
BBa_K658002	.	CDS	1519	2265	.	+	0	ID=annotation2083047;Name=pcyA;Parent=BBa_K322123
BBa_K658002	.	ribosome_entry_site	912	923	.	+	0	ID=annotation2083044;Name=B0032;Parent=BBa_K322123
BBa_K658002	.	promoter	870	904	.	+	0	ID=annotation2083043;Name=J23110;Parent=BBa_K322123
BBa_K658002	.	promoter	4538	4645	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_K568000
BBa_K658002	.	non_covalent_binding_site	4571	4588	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K658002	.	non_covalent_binding_site	4591	4608	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K658002	.	promoter	4635	4640	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K658002	.	promoter	4612	4617	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K658002	.	non_covalent_binding_site	4550	4567	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K658002	.	engineered_region	2277	4529	.	+	0	ID=BBa_K322124;Name=BBa_K322124;Parent=BBa_K568000
BBa_K658002	.	ribosome_entry_site	2282	2286	.	+	0	ID=annotation2083481;Name=RBS;Parent=BBa_K322124
BBa_K658002	.	CDS	2292	4526	.	+	0	ID=annotation2083482;Name=Cph8;Parent=BBa_K322124
BBa_K658002	.	engineered_region	64	861	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_K658002	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_K658002	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658002	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658002	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_K658002	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658002	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658002	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658002	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658002	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_K658002	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K658002	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_K658002	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K658002	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K658002	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K658002	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K658002	.	terminator	5163	5291	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K658002	.	terminator	5163	5242	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K658002	.	engineered_region	5163	5242	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658002	.	stem_loop	5174	5217	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658002	.	terminator	5251	5291	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K658002	.	stop_codon	5284	5284	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658002	.	polyA_site	5278	5291	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658002	.	stem_loop	5258	5277	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658002	.	engineered_region	5251	5291	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K658002
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggc
aaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctg
caattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggc
aagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaat
tctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgac
gaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtgg
atgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcat
tatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtta
aagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgat
tcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggc
tacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgc
atttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacc
tttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgat
cgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccc
cctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaag
ggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcac
cgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttgggg
aagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataatactagagaggagaaaggtaccc
atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattc
agccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcatttt
agggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagt
cgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcattt
ttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcc
cttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgat
gttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtg
atgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacc
cgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcg
gttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatc
tgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgcca
ccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatt
tccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatga
ccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggc
catttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttg
gagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccg
tggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggt
taattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcc
tttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctga
aaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatga
ccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatg
agcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcg
actacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccga
aagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaa
cgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccga
atcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgt
ccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataac
gggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgc
agggcatgacaaaagaagggtaataatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaac
atcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagattaaaga
ggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtt
acccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgccctt
cccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgt
ggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagat
aaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtg
tgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgat
gttctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata