##gff-version 3
##sequence-region BBa_K654051 1 2993
BBa_K654051	.	engineered_region	1	1845	.	+	0	ID=BBa_K654043;Name=BBa_K654043
BBa_K654051	.	ribosome_entry_site	1	49	.	+	0	ID=BBa_K390001;Name=BBa_K390001;Parent=BBa_K654043
BBa_K654051	.	sequence_feature	1	49	.	+	0	ID=annotation2076272;Name=5' UTR with native RBS;Parent=BBa_K390001
BBa_K654051	.	ribosome_entry_site	33	42	.	+	0	ID=annotation2076274;Name=native RBS;Parent=BBa_K390001
BBa_K654051	.	ribosome_entry_site	35	38	.	+	0	ID=annotation2076273;Name=SDS core consensus;Parent=BBa_K390001
BBa_K654051	.	engineered_region	56	1845	.	+	0	ID=BBa_K654033;Name=BBa_K654033;Parent=BBa_K654043
BBa_K654051	.	terminator	1717	1845	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K654033
BBa_K654051	.	terminator	1717	1796	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K654051	.	stem_loop	1728	1771	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K654051	.	engineered_region	1717	1796	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K654051	.	terminator	1805	1845	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K654051	.	engineered_region	1805	1845	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K654051	.	stop_codon	1838	1838	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K654051	.	stem_loop	1812	1831	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K654051	.	polyA_site	1832	1845	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K654051	.	CDS	56	1708	.	+	0	ID=BBa_I712019;Name=BBa_I712019;Parent=BBa_K654033
BBa_K654051	.	engineered_region	1854	2993	.	+	0	ID=BBa_K654038;Name=BBa_K654038
BBa_K654051	.	engineered_region	1921	2993	.	+	0	ID=BBa_K654034;Name=BBa_K654034;Parent=BBa_K654038
BBa_K654051	.	engineered_region	1921	2856	.	+	0	ID=BBa_J52008;Name=BBa_J52008;Parent=BBa_K654034
BBa_K654051	.	CDS	1921	2856	.	+	0	ID=annotation1902845;Name=Rluc;Parent=BBa_J52008
BBa_K654051	.	engineered_region	1921	2856	.	+	0	ID=annotation1902833;Name=Rluc;Parent=BBa_J52008
BBa_K654051	.	terminator	2865	2993	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K654034
BBa_K654051	.	terminator	2865	2944	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K654051	.	stem_loop	2876	2919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K654051	.	engineered_region	2865	2944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K654051	.	terminator	2953	2993	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K654051	.	engineered_region	2953	2993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K654051	.	stop_codon	2986	2986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K654051	.	stem_loop	2960	2979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K654051	.	polyA_site	2980	2993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K654051	.	promoter	1854	1914	.	+	0	ID=BBa_K390018;Name=BBa_K390018;Parent=BBa_K654038
BBa_K654051	.	sequence_feature	1862	1867	.	+	0	ID=annotation2076611;Name=-35 box;Parent=BBa_K390018
BBa_K654051	.	promoter	1857	1898	.	+	0	ID=annotation2076608;Name=sigA promoter;Parent=BBa_K390018
BBa_K654051	.	ribosome_entry_site	1899	1908	.	+	0	ID=annotation2076609;Name=putative rbs;Parent=BBa_K390018
BBa_K654051	.	sequence_feature	1899	1899	.	+	0	ID=annotation2076610;Name=+1 Transcription Start Point;Parent=BBa_K390018
BBa_K654051	.	sequence_feature	1883	1888	.	+	0	ID=annotation2076612;Name=-10 box;Parent=BBa_K390018
>BBa_K654051
agtcagttccaatctgaacatcgacaaatacataaggaattataaccaatactagatggaagacgccaaaaacataaag
aaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccc
tggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgt
tcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaa
ttctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaat
tgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgca
aaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacg
ttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattg
cactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagatt
ctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcac
ggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagc
tgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaag
cactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggg
gaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctga
ttacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctgga
taccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaac
aatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacg
aacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatc
catcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgcc
gccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccg
cgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaat
cagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagcctcaactttgactgaccagctaattttgtacacgacttagga
gttaacgaggaaaacggctactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctc
agtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaa
cgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggct
agatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcact
acaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttg
tctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatc
gagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttg
agaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacct
ggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggc
aagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcg
agtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaa
gggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaag
aacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata