##gff-version 3
##sequence-region BBa_K640006 1 4066
BBa_K640006	.	promoter	1	178	.	+	0	ID=BBa_K640008;Name=BBa_K640008
BBa_K640006	.	oriT	3425	4066	.	+	0	ID=BBa_K640004;Name=BBa_K640004
BBa_K640006	.	oriT	3807	4066	.	+	0	ID=BBa_K640002;Name=BBa_K640002;Parent=BBa_K640004
BBa_K640006	.	sequence_feature	3425	3798	.	+	0	ID=BBa_K640003;Name=BBa_K640003;Parent=BBa_K640004
BBa_K640006	.	engineered_region	187	3416	.	+	0	ID=BBa_I732019;Name=BBa_I732019
BBa_K640006	.	terminator	3288	3367	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732019
BBa_K640006	.	stem_loop	3299	3342	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K640006	.	engineered_region	3288	3367	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K640006	.	CDS	205	3279	.	+	0	ID=BBa_I732005;Name=BBa_I732005;Parent=BBa_I732019
BBa_K640006	.	point_mutation	3228	3228	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K640006	.	stop_codon	3277	3279	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K640006	.	start_codon	205	207	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_K640006	.	CDS	205	3279	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_K640006	.	ribosome_entry_site	187	198	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732019
BBa_K640006	.	conserved_region	191	194	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K640006	.	terminator	3376	3416	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732019
BBa_K640006	.	stop_codon	3409	3409	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K640006	.	engineered_region	3376	3416	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K640006	.	stem_loop	3383	3402	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K640006	.	polyA_site	3403	3416	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K640006
aaacgctcctgtttatgtgtgtcctgcggtgaaacggctgcgcaatccagtataggcagtgctaaaagtgaggctatgg
cgattttcaggtcactccgggctttcttgacaaggaaaaagactggtatgagttcagagacccgcgccgtctcggccga
ataaaacaggaatcagcacctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgtt
ttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggc
gtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtt
tccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaac
tggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttccca
cggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattat
ttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccg
tctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggca
gttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacaca
aatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgc
ggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctt
tcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaact
gtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaa
gcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgag
gcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgat
gaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctac
ggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgct
ggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgct
ggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtg
cagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagc
ccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcga
atacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacag
ggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcg
gtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagc
gctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatac
ctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgc
ctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctg
gctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgt
ctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggattttt
gcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaa
acaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgc
attgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacgg
cagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccg
gaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccg
gcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaact
atcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccccgtacgtcttccc
gagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaac
atcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctga
atatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatttcagctgagcgc
cggtcgctaccattaccagttggtctggtgtcaaaaataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagcgccgctggtgccgctggttggacgccaagggtgaatccgcctcgataccct
gattactcgcttcctgcgccctctcaggcggcgataggggactggtaaaacggggattgcccagacgcctcccccgccc
cttcaggggcacaaatgcggccccaacggggccacgtagtggtgcgttttttgcgtttccacccttttcttccttttcc
cttttaaaccttttaggacgtctacaggccacgtaatccgtggcctgtagagtttaaaaagggacggatttgttgccat
taagggacggatttgttgttaagaagggacggatttgttgttgtaaagggacggatttgttgtattgtgggacgcagat
acagtgtactagagttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatc
catcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagcc
gggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctc
aacgggaatcctgctctgcgaggctggccgataagct