##gff-version 3
##sequence-region BBa_K614000 1 882
BBa_K614000	.	engineered_region	109	882	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K614000	.	CDS	130	835	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K614000	.	CDS	130	804	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K614000	.	engineered_tag	811	835	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K614000	.	ribosome_entry_site	109	123	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K614000	.	ribosome_entry_site	109	123	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K614000	.	engineered_region	109	123	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K614000	.	ribosome_entry_site	116	120	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K614000	.	terminator	844	882	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K614000	.	polyA_site	844	852	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K614000	.	polyA_site	875	882	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K614000	.	engineered_region	844	882	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K614000	.	stem_loop	853	874	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K614000	.	promoter	1	100	.	+	0	ID=BBa_K216005;Name=BBa_K216005
BBa_K614000	.	non_covalent_binding_site	89	94	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
BBa_K614000	.	non_covalent_binding_site	50	65	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
BBa_K614000	.	non_covalent_binding_site	66	71	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K614000	.	non_covalent_binding_site	58	80	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
>BBa_K614000
ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaat
tatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaaga
gttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgt
ccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccc
cgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgga
aggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagac
ggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgg
gttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaa
agacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaa
accgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtc
actccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacaggg
cggggtttttttt