##gff-version 3
##sequence-region BBa_K581003 174 364
BBa_K581003	.	point_mutation	176	176	.	+	0	ID=annotation2152333;Name=G176C mutant of SgrS(wt)
BBa_K581003	.	engineered_region	236	364	.	+	0	ID=annotation2152345;Name=B0015
BBa_K581003	.	conserved_region	174	179	.	+	0	ID=annotation2152332;Name=critical region for SgrS/ptsG interaction
>BBa_K581003
gatgaagcaagggggtgccccatgcgtcagttttatcagcactattttaccgcgacagcgaagttgtgctggttgcgtt
ggttaagcgtcccacaacgattaaccatgcttgaaggactgatgcagtgggatgaccgcaattctgaaagttgacttgc
ctgcatcatgtgtgactgactattggtgtaaaatcacccgccagcagattatacctgctggtttttttttactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata