##gff-version 3
##sequence-region BBa_K575027 1 1684
BBa_K575027	.	engineered_region	901	1684	.	+	0	ID=BBa_K575005;Name=BBa_K575005
BBa_K575027	.	promoter	901	935	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K575005
BBa_K575027	.	ribosome_entry_site	944	955	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K575005
BBa_K575027	.	conserved_region	948	951	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K575027	.	CDS	962	1684	.	+	0	ID=BBa_C0179;Name=BBa_C0179;Parent=BBa_K575005
BBa_K575027	.	engineered_region	1	892	.	+	0	ID=BBa_K575010;Name=BBa_K575010
BBa_K575027	.	ribosome_entry_site	166	180	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575010
BBa_K575027	.	engineered_region	166	180	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K575027	.	ribosome_entry_site	173	177	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K575027	.	ribosome_entry_site	166	180	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K575027	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079;Parent=BBa_K575010
BBa_K575027	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K575027	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K575027	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K575027	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K575027	.	CDS	187	892	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575010
BBa_K575027	.	engineered_tag	868	892	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K575027	.	CDS	187	861	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K575027
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccg
ctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaa
agcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgtt
atgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagtta
aactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacg
tatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgct
gaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatca
cctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacg
ctgatagtgctagtgtagatcgctactagagttgacagctagctcagtcctaggtataatgctagctactagagaaaga
ggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatc
ctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgaga
acgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgac
ggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttc
ttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgc
tgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgct
caaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgg
gagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagcca
atgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaa
tttgggtcttattactctctaataa