##gff-version 3
##sequence-region BBa_K569015 1 2467
BBa_K569015	.	engineered_region	1	1031	.	+	0	ID=BBa_J07011;Name=BBa_J07011
BBa_K569015	.	ribosome_entry_site	154	168	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07011
BBa_K569015	.	ribosome_entry_site	154	168	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K569015	.	engineered_region	154	168	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K569015	.	ribosome_entry_site	161	165	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K569015	.	terminator	903	982	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J07011
BBa_K569015	.	stem_loop	914	957	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K569015	.	engineered_region	903	982	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K569015	.	promoter	1	145	.	+	0	ID=BBa_J07007;Name=BBa_J07007;Parent=BBa_J07011
BBa_K569015	.	sequence_feature	133	138	.	+	0	ID=annotation1579234;Name=-10 region;Parent=BBa_J07007
BBa_K569015	.	promoter	1	145	.	+	0	ID=annotation1571612;Name=ctx promoter;Parent=BBa_J07007
BBa_K569015	.	sequence_feature	145	145	.	+	0	ID=annotation1577387;Name=transcription start;Parent=BBa_J07007
BBa_K569015	.	terminator	991	1031	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J07011
BBa_K569015	.	stem_loop	998	1017	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K569015	.	stop_codon	1024	1024	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K569015	.	polyA_site	1018	1031	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K569015	.	engineered_region	991	1031	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K569015	.	CDS	175	894	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J07011
BBa_K569015	.	CDS	175	894	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K569015	.	engineered_region	1038	2467	.	+	0	ID=BBa_K569012;Name=BBa_K569012
BBa_K569015	.	CDS	1038	1667	.	+	0	ID=BBa_J07009;Name=BBa_J07009;Parent=BBa_K569012
BBa_K569015	.	sequence_feature	1038	1583	.	+	0	ID=annotation1668506;Name=Cytoplasmic Region;Parent=BBa_J07009
BBa_K569015	.	start_codon	1043	1045	.	+	0	ID=annotation1668508;Name=Start Codon;Parent=BBa_J07009
BBa_K569015	.	CDS	1038	1667	.	+	0	ID=annotation1668507;Name=Coding Sequence for ToxR';Parent=BBa_J07009
BBa_K569015	.	sequence_feature	1584	1631	.	+	0	ID=annotation1668509;Name=Transmembrane Region;Parent=BBa_J07009
BBa_K569015	.	sequence_feature	1632	1667	.	+	0	ID=annotation1668510;Name=Periplasmic Region;Parent=BBa_J07009
BBa_K569015	.	CDS	1676	2467	.	+	0	ID=BBa_K569011;Name=BBa_K569011;Parent=BBa_K569012
>BBa_K569015
aacagaaaatgataaaaaaggactaatagtatattttgatttttgatttttgatttttgatttttgatttttgattttt
gatttttgatttcaaataatacaaatttatttacttatttaattgttttgatcaattatttttctgtactagagattaa
agaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgat
gttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttattt
gcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagata
cccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaag
gtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcat
ggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagca
gaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctg
ccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatgg
catggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgct
gaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagca
acgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttg
gcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcg
acaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaag
agatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgt
gccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaat
cgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaac
ccctaacgtactagagccgtccccgaccttgcctgaacaagatgctctcccctcctcggaggatgatgatgatgatgat
gactcctcttcagaggagaaagaaacagataacaccaaaccaaaccccgtagctccatattggacatccccagaaaaga
tggaaaagaaattgcatgcagtgccggctgccaagacagtgaagttcaaatgcccttccagtgggaccccaaaccccac
actgcgctggttgaaaaatggcaaagagttcaaacctgaccacagaattggaggctacaaggtccgttatgccacctgg
agcatcataatggactctgtggtgccctctgacaagggcaactacacctgcattgtggagaatgagtacggcagcatca
accacacataccagctggatgtcgtggagcggtcccctcaccggcccatcctgcaagcagggttgcccgccaacaaaac
agtggccctgggtagcaacgtggagttcatgtgtaaggtgtacagtgacccgcagccgcacatccagtggctaaagcac
atcgaggtgaatgggagcaagattggcccagacaacctgccttatgtccagatcttgaagactgctggagttaatacca
ccgacaaagagatggaggtgcttcacttaagaaatgtctcctttgaggacgcaggggagtatacgtgcttggcgggtaa
ctctatcggactctcccatcactctgcatggttgaccgttctggaagccctggaagagaggccggcagtgatgacctcg
cccctgtacctggagatc