LOCUS       BBa_K567025             2055 bp    DNA     linear       09-Dec-2025
DEFINITION  PT7-Luc-8AGG-tRNA(Arg)
ACCESSION   BBa_K567025
VERSION     BBa_K567025.1  
FEATURES             Location/Qualifiers
     promoter        2..18
                     /label=PT7
     misc_feature    21..44
                     /label=lac operator
     RBS             75..81
                     /label=rbs
     misc_feature    89..91
                     /label=ATG
     CDS             89..1774
                     /label=luciferase
     misc_feature    95..118
                     /label=8AGG
     misc_feature    1881..1955
                     /label=tRNA(Arg)
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat ggcgaggagg aggaggagga ggaggagggc
      121 ggcggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      181 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      241 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      301 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      361 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      421 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
      481 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      541 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
      601 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      661 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
      721 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
      781 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
      841 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
      901 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
      961 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
     1021 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
     1081 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     1141 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     1201 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     1261 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     1321 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     1381 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     1441 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     1501 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     1561 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     1621 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     1681 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     1741 aaaggccaag aagggcggaa agatcgccgt gtaacatatg gaaaaattat aaaaacccgg
     1801 cataaatggc gagggtttaa gcaatcgagc ggcagcgtac ttaccccgca ctccattagc
     1861 gggtatactc atgccgcatt gtcctcttag ttaaatggat ataacgagcc cctcctaagg
     1921 gctaattgca ggttcgattc ctgcagggga caccatttat cagttcgctc ccatccgtac
     1981 cagtccgcaa aatcccctga atatcaagca ttccgtagat ttacagttcg tcatggttcg
     2041 cttcagatcg ttgac
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