##gff-version 3
##sequence-region BBa_K543001 1 615
BBa_K543001	.	terminator	487	566	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K543001	.	engineered_region	487	566	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543001	.	stem_loop	498	541	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543001	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K543001	.	CDS	65	478	.	+	0	ID=BBa_K543000;Name=BBa_K543000
BBa_K543001	.	engineered_region	65	478	.	+	0	ID=annotation2153075;Name=HNS protein;Parent=BBa_K543000
BBa_K543001	.	sequence_alteration	466	466	.	+	0	ID=annotation2153076;Name=Change XbaI site into no-coding;Parent=BBa_K543000
BBa_K543001	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K543001	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K543001	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K543001	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K543001	.	terminator	575	615	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K543001	.	stop_codon	608	608	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543001	.	polyA_site	602	615	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543001	.	stem_loop	582	601	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543001	.	engineered_region	575	615	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K543001
tttacagctagctcagtcctaggtattatgctagctactagagattaaagaggagaaatactagatgagcgaagcactt
aaaattctgaacaacatccgtactcttcgtgcgcaggcaagagaatgtacacttgaaacgctggaagaaatgctggaaa
aattagaagttgttgttaacgaacgtcgcgaagaagaaagcgcggctgctgctgaagttgaagagcgcactcgtaaact
gcagcaatatcgcgaaatgctgatcgctgacggtattgacccgaacgaactgctgaatagccttgctgccgttaaatct
ggcaccaaagctaaacgtgctcagcgtccggcaaaatatagctacgttgacgaaaacggcgaaactaaaacctggactg
gccaaggccgtactccagctgtaatcaaaaaagcaatggatgagcaaggtaaatccctcgacgatttcctgatcaagca
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata