##gff-version 3
##sequence-region BBa_K539691 1 6200
BBa_K539691	.	engineered_region	1	3448	.	+	0	ID=BBa_K539651;Name=BBa_K539651
BBa_K539691	.	engineered_region	1	1943	.	+	0	ID=BBa_K539631;Name=BBa_K539631;Parent=BBa_K539651
BBa_K539691	.	engineered_region	209	1943	.	+	0	ID=BBa_K539627;Name=BBa_K539627;Parent=BBa_K539631
BBa_K539691	.	CDS	228	1943	.	+	0	ID=BBa_K539713;Name=BBa_K539713;Parent=BBa_K539627
BBa_K539691	.	CDS	228	1943	.	+	0	ID=annotation2136210;Name=AlsS;Parent=BBa_K539713
BBa_K539691	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K539627
BBa_K539691	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K539691	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K539691	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K539691	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K539631
BBa_K539691	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K539691	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K539691	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K539691	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K539691	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K539691	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K539691	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K539691	.	engineered_region	1952	3448	.	+	0	ID=BBa_K539621;Name=BBa_K539621;Parent=BBa_K539651
BBa_K539691	.	CDS	1973	3448	.	+	0	ID=BBa_K539711;Name=BBa_K539711;Parent=BBa_K539621
BBa_K539691	.	CDS	1973	3448	.	+	0	ID=annotation2136206;Name=ilvC coding sequence;Parent=BBa_K539711
BBa_K539691	.	ribosome_entry_site	1952	1966	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K539621
BBa_K539691	.	ribosome_entry_site	1952	1966	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K539691	.	ribosome_entry_site	1959	1963	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K539691	.	engineered_region	1952	1966	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K539691	.	engineered_region	3457	6200	.	+	0	ID=BBa_K539653;Name=BBa_K539653
BBa_K539691	.	engineered_region	3457	5328	.	+	0	ID=BBa_K539624;Name=BBa_K539624;Parent=BBa_K539653
BBa_K539691	.	ribosome_entry_site	3457	3471	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K539624
BBa_K539691	.	ribosome_entry_site	3457	3471	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K539691	.	ribosome_entry_site	3464	3468	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K539691	.	engineered_region	3457	3471	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K539691	.	CDS	3478	5328	.	+	0	ID=BBa_K539712;Name=BBa_K539712;Parent=BBa_K539624
BBa_K539691	.	CDS	3478	5328	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K539691	.	engineered_region	5337	6200	.	+	0	ID=BBa_K332033;Name=BBa_K332033;Parent=BBa_K539653
BBa_K539691	.	terminator	6088	6200	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332033
BBa_K539691	.	mature_transcript_region	5337	5388	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332033
BBa_K539691	.	stem_loop	5360	5388	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539691	.	sequence_alteration	5359	5364	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539691	.	ribosome_entry_site	5383	5388	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539691	.	stem_loop	5337	5354	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539691	.	CDS	5395	6079	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332033
BBa_K539691	.	CDS	5398	6014	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K539691	.	tag	6015	6048	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K539691	.	engineered_tag	6055	6079	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_K539691
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgttgacaa
aagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgt
cacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatc
gttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttag
tcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgct
tgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgatt
acaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcag
ggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgt
tgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcct
gtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttc
catttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgtt
ccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgat
ccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttacc
agcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgc
agagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatca
gacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcg
gttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaac
actcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgac
ggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacg
acagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcga
tatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgt
caaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgaca
agcttccgaaagaatttggggaactcatgaaaacgaaagctctctagtactagagattaaagaggagaaatactagatg
gctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaat
ttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcct
gaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgt
aaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgc
cggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacgg
tttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcacc
gaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcg
aaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgc
ggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgac
aagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcac
tgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaaca
gctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatg
gcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgc
agtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttga
actggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctg
attgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatc
tgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattcc
ggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaag
aaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatactagagattaaagaggagaaatacta
gatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccgga
atgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatc
tgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggt
ggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgag
tatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatgg
cttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatca
gatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggtt
gaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgc
tgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaa
acgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcg
gcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgc
aggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgcc
gagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcg
gggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctga
cccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattg
ccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggc
ctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaat
tcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggaga
tgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaa
tcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggcc
acgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaa
ccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcc
tggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaag
gcgcggtgcgcgataaatcgaaactggggggttaatactagagggacaagcaatgcttgccttgaatagtaacttttga
atagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgag
gtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaa
aaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagg
ggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaa
gtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtt
tttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagca
tcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattattt
gatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtg
aaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactact
agagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaa
agatgaatgactgtccacgacgctatacccaaaagaaa