##gff-version 3
##sequence-region BBa_K538210 1 2199
BBa_K538210	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K538210	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K538210	.	CDS	550	2199	.	+	0	ID=BBa_K538001;Name=BBa_K538001
BBa_K538210	.	point_mutation	1457	1457	.	+	0	ID=annotation2128866;Name=A > G (Recode for SapI site);Parent=BBa_K538001
BBa_K538210	.	stop_codon	2194	2199	.	+	0	ID=annotation2124232;Name=Double Stop Codon;Parent=BBa_K538001
BBa_K538210	.	start_codon	550	552	.	+	0	ID=annotation2124231;Name=Start Codon;Parent=BBa_K538001
BBa_K538210	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K538210	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K538210	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K538210	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K538210	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K538210	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K538210	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K538210	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K538210	.	ribosome_entry_site	532	543	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K538210	.	conserved_region	536	539	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K538210	.	CDS	227	523	.	+	0	ID=BBa_K538000;Name=BBa_K538000
BBa_K538210	.	start_codon	227	229	.	+	0	ID=annotation2124174;Name=Start Codon;Parent=BBa_K538000
BBa_K538210	.	stop_codon	518	523	.	+	0	ID=annotation2124175;Name=Double Stop Codon;Parent=BBa_K538000
>BBa_K538210
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaatccg
tccattacatgatcgtattgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattattttaccgggcgct
gcggcagaaaaaccaaatcaaggtgttgttatctctgtgggtactggccgtattcttgataatggttcagtgcaagcgc
tggcggttaacgaaggcgatgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatggtgaagaatt
attgattttgaatgaaagtgatatctacggcgttttagaagcttaataatactagagaaagaggagaaatactagatgg
ctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaag
agttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtt
tctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaag
ccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgt
tgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaa
caagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtc
gtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacga
gcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgacc
gtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaa
acgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaa
caacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagat
cttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgg
gtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatac
tcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgct
aagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttg
acgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcact
ctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcct
atccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttg
gttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatc
tctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggc
gctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa