##gff-version 3
##sequence-region BBa_K525562 1 2410
BBa_K525562	.	sequence_feature	1129	1134	.	+	0	ID=annotation2161138;Name=Freiburg Scar
BBa_K525562	.	stop_codon	2408	2410	.	+	0	ID=annotation2160994;Name=TAA
BBa_K525562	.	promoter	1	35	.	+	0	ID=annotation2160997;Name=promoter
BBa_K525562	.	CDS	818	1128	.	+	0	ID=annotation2161293;Name=BisdA
BBa_K525562	.	non_covalent_binding_site	2402	2407	.	+	0	ID=annotation2160993;Name=AgeI
BBa_K525562	.	ribosome_entry_site	47	50	.	+	0	ID=annotation2161296;Name=RBS
BBa_K525562	.	engineered_region	44	55	.	+	0	ID=annotation2160998;Name=B0034
BBa_K525562	.	non_covalent_binding_site	65	70	.	+	0	ID=annotation2160995;Name=NgoMIV
BBa_K525562	.	start_codon	62	64	.	+	0	ID=annotation2160996;Name=ATG
BBa_K525562	.	CDS	162	811	.	+	0	ID=annotation2161295;Name=Reductase
BBa_K525562	.	engineered_region	62	811	.	+	0	ID=annotation2160999;Name=K525499
BBa_K525562	.	sequence_feature	812	817	.	+	0	ID=annotation2161292;Name=Freiburg Scar
BBa_K525562	.	CDS	1135	2410	.	+	0	ID=annotation2161294;Name=BisdB
BBa_K525562	.	engineered_region	1135	2410	.	+	0	ID=annotation2161001;Name=K123001
BBa_K525562	.	engineered_region	818	1128	.	+	0	ID=annotation2161000;Name=K123000
>BBa_K525562
tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggccggcgctgattgg
gtaacaggcaaagtcactaaagtgcagaactggaccgacgccctgtttagtctcaccgttcacgcccccgtgcttccgt
ttaccgccgggcaatttaccaagcttggccttgaaatcgacggcgaacgcgtccagcgcgcctactcctatgtaaactc
gcccgataatcccgatctggagttttacctggtcaccgtccccgatggcaaattaagcccacgactggcggcactgaaa
ccaggcgatgaagtgcaggtggttagcgaagcggcaggattctttgtgctcgatgaagtgccgcactgcgaaacgctat
ggatgctggcaacgggcacagcgattggcccttatttatcgattctgcaactaggtaaagatttagatcgcttcaaaaa
tctggtcctggtgcacgccgcacgttatgccgccgacttaagctatttgccactgatgcaggaactggaaaaacgctac
gaaggaaaactgcgcattcagacggtggtcagtcgggaaacggcagcggggtcgctcaccggacggataccggcattaa
ttgaaagtggggaactggaaagcacgattggcctgccgatgaataaagaaaccagccatgtgatgctgtgcggcaatcc
acagatggtgcgcgatacacaacagttgctgaaagagacccggcagatgacgaaacatttacgtcgccgaccgggccat
atgacagcggagcattactggaccggccctcatatccaagttacgactcgtgatggtgaaattcgcgagctggacgttg
cagcttccggtttcctgatggaagcgctgcgtgacgctaacatcgatggtgtggaggcgatgtgtggcggttgctgttc
ttgcgcgacttgccacgtctacattgacgcggctccagccggtaccctgccgccggtgtctagcgatgaagaaatgctg
ctgtccggcctggtttctaccccgggccgctctcgtctgtcttgccagatcccggtaaccgcagaactggacggcctga
aactgaccatcccgccggatagcaccggcaacccacaaactctgccggtatttccagacctggatatcttcagcccgga
atacgcttgtaaccgcgaaaaatatgcagcgcgtgcgctgcgcgattacccgctgcacttttataaaccgctgaacctg
tggattgtctccaaacataaagacgttcgtagcgcgctgttcaccccgcaggtcttcagctccgtagcttttggtctgc
tgcctccaccagacgatatcgctcctcgcgtgccggacctgtacaccgacgttcatctgccgtccatggatccgccgga
gcacaccaaactgcgtgttccggtgcagcaggctctgctgccgggccgtctggtaggcaaagacgaggttgtgcgccgt
attgcgaacgaactgatcgatacttttatcgacaaaggcgaatgcgatctgctgcatgatttctcttataagctggcgc
tgtacctgattgtagacctgctgggtatccctaaagaacgtgcggaggactaccaccgctggtccaactgtttcttcca
gctgttcacgccgaaggttcctgaacgtgcggatgctcgtttctttgtaccgatgccggaggaagtgctgcgccagaac
tgggaaggtctggccgaagcaaatgactatctgcgtgaagttgttgaaaacctggaccgtaatccgggcaacaacatgc
tgtctaacctgctgcaactgcgtgaaccggatggctctcgtactatcactatctccgcgaacgtttgcaacgccctgga
atttggcgcggcgggtcacgatacgaccgcaaccctgatcgctcacctgacttatttcgtgctgaccaccccggacctg
aaggacactctgaccgaagatccatctctgattccggcagccatctctgaaaccctgcgtcgtcgttgcccggttgacg
gtctgttccgccgcaccctgagcgacgtagaactgtgcggtcagaagatcgaatccggttctatcgtgtacctggacct
gaccgccgctaacctggatccggacgttttcccggagccggagactttccgtctgaaccgcgataacattaaagaaatg
gtgtctttcggcttcggtcgtcacgtctgtgcaggtcagtacctgagccgcatcgaagctaaagcagcatacgaagaac
tgatgcgtcgtattcctaatatgcgtctggcagatggcttcaaactggagtacatgccgagcgtggccaccactgttct
gaaaggcctgccgctggtttgggataaaaacaccggttaa