##gff-version 3
##sequence-region BBa_K510021 1 12194
BBa_K510021	.	plasmid	1	4549	.	+	0	ID=BBa_K510012;Name=BBa_K510012
BBa_K510021	.	primer_binding_site	157	176	.	+	0	ID=annotation2131786;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	3490	3537	.	+	0	ID=annotation2131802;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510021	.	CDS	3554	4087	.	+	0	ID=annotation2131805;Name=Gentamicin resistance;Parent=BBa_K510012
BBa_K510021	.	engineered_region	3385	3479	.	+	0	ID=annotation2131801;Name=double terminator (BBa_B0014);Parent=BBa_K510012
BBa_K510021	.	sequence_feature	4345	4350	.	+	0	ID=annotation2131807;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	348	360	.	+	0	ID=annotation2131798;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510021	.	primer_binding_site	4410	4429	.	+	0	ID=annotation2131841;Name=VF2 (G00100) primer binding site;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	4357	4404	.	+	0	ID=annotation2131856;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510021	.	sequence_feature	646	905	.	+	0	ID=annotation2131797;Name=oriT;Parent=BBa_K510012
BBa_K510021	.	stem_loop	22	93	.	+	0	ID=annotation2131863;Name=E. coli his operon terminator;Parent=BBa_K510012
BBa_K510021	.	engineered_region	4528	4549	.	+	0	ID=annotation2131839;Name=Prefix;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	3542	3547	.	+	0	ID=annotation2131806;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	929	1310	.	+	0	ID=annotation2131796;Name=R6K replication origin;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	4351	4355	.	+	0	ID=annotation2131808;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	3163	3175	.	+	0	ID=annotation2131803;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	3548	3553	.	+	0	ID=annotation2131804;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510021	.	stem_loop	4487	4520	.	+	0	ID=annotation2131866;Name=Terminator;Parent=BBa_K510012
BBa_K510021	.	engineered_region	2	21	.	+	0	ID=annotation2131765;Name=Suffix;Parent=BBa_K510012
BBa_K510021	.	sequence_feature	3176	3374	.	+	0	ID=annotation2131800;Name=Tn7-R end;Parent=BBa_K510012
BBa_K510021	.	CDS	1714	2574	.	+	0	ID=annotation2131795;Name=Ampicillin resistance (bla);Parent=BBa_K510012
BBa_K510021	.	sequence_feature	182	347	.	+	0	ID=annotation2131766;Name=Tn7-L end;Parent=BBa_K510012
BBa_K510021	.	engineered_region	4558	12194	.	+	0	ID=BBa_K325909;Name=BBa_K325909
BBa_K510021	.	CDS	10383	11480	.	+	0	ID=annotation2112058;Name=luxE;Parent=BBa_K325909
BBa_K510021	.	CDS	8186	9250	.	+	0	ID=annotation2112056;Name=luxA;Parent=BBa_K325909
BBa_K510021	.	CDS	9289	10269	.	+	0	ID=annotation2112057;Name=luxB;Parent=BBa_K325909
BBa_K510021	.	CDS	11484	12194	.	+	0	ID=annotation2112059;Name=luxG;Parent=BBa_K325909
BBa_K510021	.	CDS	5788	7217	.	+	0	ID=annotation2112039;Name=luxC;Parent=BBa_K325909
BBa_K510021	.	ribosome_entry_site	8173	8178	.	+	0	ID=annotation2112117;Name=putative rbs;Parent=BBa_K325909
BBa_K510021	.	ribosome_entry_site	11472	11476	.	+	0	ID=annotation2112122;Name=putative rbs;Parent=BBa_K325909
BBa_K510021	.	CDS	7232	8135	.	+	0	ID=annotation2112076;Name=luxD;Parent=BBa_K325909
BBa_K510021	.	promoter	5600	5671	.	+	0	ID=annotation2112036;Name=PC;Parent=BBa_K325909
BBa_K510021	.	ribosome_entry_site	10332	10338	.	+	0	ID=annotation2112119;Name=putative rbs;Parent=BBa_K325909
BBa_K510021	.	ribosome_entry_site	9276	9281	.	+	0	ID=annotation2112062;Name=putative rbs;Parent=BBa_K325909
BBa_K510021	.	ribosome_entry_site	7221	7225	.	+	0	ID=annotation2112061;Name=putative rbs;Parent=BBa_K325909
BBa_K510021	.	sequence_feature	4558	5636	.	+	0	ID=annotation2111897;Name=AraC;Parent=BBa_K325909
BBa_K510021	.	ribosome_entry_site	5768	5787	.	+	0	ID=annotation2112060;Name=putative rbs region;Parent=BBa_K325909
BBa_K510021	.	sequence_feature	5637	5767	.	+	0	ID=annotation2112038;Name=pBAD;Parent=BBa_K325909
>BBa_K510021
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatg
agtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgct
cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatcc
atcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccg
ggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctca
acgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgata
gtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaac
cctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaa
caataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataagg
cttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctc
aaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa
gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagt
gctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
cctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtct
gtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactat
gcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc
gcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgcca
gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtctta
aactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaa
gcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
tttgctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggtt
aggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcggg
atcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagc
gcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaa
acttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttact
acggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaa
agatcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccac
ctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatca
aacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagc
catgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctac
ttgcattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttc
catgggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtct
aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcctt
agctttcgctaaggatgatttctggaattcgcggccgcttctagagtactagagttatgacaacttgacggctacatca
ttcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaataga
gttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggct
gatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgac
aagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgc
gtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatt
tatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggc
tggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgc
gcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgg
gaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgaga
ttgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaac
ccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactccc
gccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgcta
accaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaa
gtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatc
cataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
agggaaaataatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaa
gttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaag
atagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataa
tcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattgg
attgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatg
aatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttcc
attatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatcct
tttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgt
attggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggaga
cgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagt
attattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcct
gcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcg
atacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtcta
tttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatc
aaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaac
acaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaatt
gttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggt
ttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagagga
agataagtttttagtttttgtcccatagttaaaggaaattatatgaaagatgaaagtgctttttttacgattgatcaca
tcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatac
aatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggtttt
catgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaa
aaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtc
agcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttaga
gatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaag
gtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatag
tgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagag
ctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttgg
ttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagataga
cattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaa
ttattagaattggcttaaataaacagaatcaccaaaaaggaatagagtatgaagtttggaaatatttgtttttcgtatc
aaccaccaggtgaaactcataagcaagtaatggatcgctttgttcggcttggtatcgcctcagaagaggtagggtttga
tacatattggaccttagaacatcattttacagagtttggtcttacgggaaatttatttgttgctgcggctaacctgtta
ggaagaactaaaacattaaatgttggcactatgggggttgttattccgacagcacacccagttcgacagttagaagacg
ttttattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcgagggctataccataaagattttcgagt
atttggtgttgatatggaagagtctcgagcaattactcaaaatttctaccagatgataatggaaagcttacagacagga
accattagctctgatagtgattacattcaatttcctaaggttgatgtatatcccaaagtgtactcaaaaaatgtaccaa
cctgtatgactgctgagtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttcttagttggattat
tggtactaatgaaaaaaaagcacagatggaactctataatgaaattgcgacagaatatggtcatgatatatctaaaata
gatcattgtatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatgtttgtcgggagtttctgaaaa
attggtatgactcatatgtaaatgcgaccaatatctttaatgatagcaatcaaactcgtggttatgattatcataaagg
tcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtgttgattatagcaatggtattaaccctgta
ggcactcctgagcagtgtattgaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatgcggatttg
aagctaatggaactgaagatgaaataattgcttccatgcgacgctttatgacacaagtcgctcctttcttaaaagaacc
taaataaattacttatttgatactagagataataaggaacaagttatgaaatttggattattttttctaaactttcaga
aagatggaataacatctgaagaaacgttggataatatggtaaagactgtcacgttaattgattcaactaaatatcattt
taatactgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagcacctattaccgcagctggtttttta
ttagggttaacaaataaattacatattggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcagaag
aagccagtttattagatcaaatgtcagagggacgcttcattcttggttttagtgactgcgaaagtgatttcgaaatgga
attttttagacgtcatatctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacgcattaactaca
ggttattgtcatccccaaaacgacttttatgattttccaaaggtttcaattaatccacactgttacagtgagaatggac
ctaagcaatatgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcactgcctttaacatttaagtg
ggaggataatttagaaaccaaagaacgctatgcaattctatataataaaacagcacaacaatatggtattgatatttcg
gatgttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagtacggctcaagaagaagtgagagaatact
taaaagactatatcactgaaacttaccctcaaatggacagagatgaaaaaattaactgcattattgaagagaatgcagt
tgggtctcatgatgactattatgaatcgacaaaattagcagtggaaaaaacagggtctaaaaatattttattatccttt
gaatcaatgtccgatattaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaatgaatttaccataat
aaaattaaaggcaatttctatattagattgcctttttaaatttctgttgatattaggtattactggagagggtatgact
gtccatactgaatataaaagaaatcaaatcattgctagttcagaaattgatgatcttatctttatgacgaaaccacaag
agtggtcatttgaagagcaaaaagaaatacgggataaattagttcgtgaggctttttattttcactacaatagaaatga
agaatatagaaattattgtatcaatcagcatgtgagtgataatttacacactattgatgaaatacccgtgtttccaaca
tctgtttttaaatataagaaattacatactgtcacagccgaggacattgaaaattggtatacaagtagtggaactcgtg
gagtaaaaagtcatattgcacgtgatcgtcttagcattgaacgcttgcttggttctgtcaacttcggaatgaaatacgt
tggagattggtttgagcatcaaatggaattgataaatttaggaccagatagattcaatacaaataatatttggtttaaa
tatgtcatgagtttggtcgagttactttatccgactgaatttacagttgataatgacaaaatagattttgaaaaaacag
taaaacatctatttagaattaagaatagtaaaaaagacatttgcttaattgggccaccattttttgtgtatcttttgtg
ccaatatatgaaagaaaacaatattgaatttaaaggaggagatagagtacatattattactggtggaggatggaaatct
aatcagaatgactctttagatcgtgctgattttaatcaattattaatggatactttccaactcgacaagattaatcaaa
ttagagatacctttaatcaagttgagcttaatacttgtttttttgaagatgaatttcaaagaaaacatgttccaccgtg
ggtatatgctcgggctcttgatcctgaaaccttgaaacccgtagcagatggtgagatcgggttgttaagttatatggac
gcctcatcaactgcttaccctgcttttattgttactgatgatatcggtattgtaaaagaaattagagaaccagatcctt
acccaggggtaactgttgagattgttcggcgcttaaatacacgtgcgcaaaaaggatgcgcgctctctatggctaatgt
catacaaaagaatatcaaggattaagttatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaat
aatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatg
ggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatag
agactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccat
ggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaata
gcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctctttt
gtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagt
gaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatattt
atgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagat
gtttgccgatgcttttgcatacgtataa