##gff-version 3
##sequence-region BBa_K415020 1 2649
BBa_K415020	.	engineered_region	1989	2649	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K415020	.	ribosome_entry_site	1989	2000	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K415020	.	conserved_region	1993	1996	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415020	.	CDS	2007	2624	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K415020	.	CDS	2007	2585	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K415020	.	engineered_tag	2625	2649	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K415020	.	engineered_region	2007	2624	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K415020	.	tag	2586	2617	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K415020	.	engineered_region	1	1980	.	+	0	ID=BBa_K415006;Name=BBa_K415006
BBa_K415020	.	engineered_region	983	1980	.	+	0	ID=BBa_S03119;Name=BBa_S03119;Parent=BBa_K415006
BBa_K415020	.	terminator	1940	1980	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K415020	.	stop_codon	1973	1973	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415020	.	polyA_site	1967	1980	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415020	.	stem_loop	1947	1966	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415020	.	engineered_region	1940	1980	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415020	.	ribosome_entry_site	1045	1056	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K415020	.	conserved_region	1049	1052	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415020	.	promoter	983	1036	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K415020	.	engineered_region	983	1036	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K415020	.	promoter	1002	1007	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K415020	.	promoter	1025	1030	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K415020	.	non_covalent_binding_site	983	1001	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K415020	.	non_covalent_binding_site	1008	1026	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K415020	.	CDS	1063	1818	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K415020	.	engineered_tag	1819	1843	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K415020	.	engineered_region	1063	1818	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K415020	.	engineered_region	1063	1064	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K415020	.	point_mutation	1236	1236	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K415020	.	CDS	1063	1812	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K415020	.	point_mutation	1554	1554	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K415020	.	terminator	1852	1931	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K415020	.	engineered_region	1852	1931	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415020	.	stem_loop	1863	1906	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415020	.	engineered_region	1	974	.	+	0	ID=BBa_K415000;Name=BBa_K415000;Parent=BBa_K415006
BBa_K415020	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K415000
BBa_K415020	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415020	.	terminator	846	925	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K415000
BBa_K415020	.	engineered_region	846	925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415020	.	stem_loop	857	900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415020	.	terminator	934	974	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K415000
BBa_K415020	.	stop_codon	967	967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415020	.	polyA_site	961	974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415020	.	stem_loop	941	960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415020	.	engineered_region	934	974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415020	.	engineered_region	124	837	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K415000
BBa_K415020	.	sequence_alteration	475	475	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K415020	.	CDS	124	834	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K415020	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K415000
BBa_K415020	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415020	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415020	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415020	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415020	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415020	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415020	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415020	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
>BBa_K415020
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccat
catcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggc
gagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggaca
tcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtc
cttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcc
ttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaaga
agaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggct
gaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggc
gcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccg
agggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagat
actgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataa
aattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattat
ttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggc
aatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattg
gaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcact
gggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactata
tagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaa
tatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagc
tcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactca
atacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaata
acactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggag
gagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacc
ttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctg
gcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaa
gatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatta
caatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagc
aatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatact
aaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacg
ctttagtagcttaataactctgatagtgctagtgtagatctc