##gff-version 3
##sequence-region BBa_K404253 2 3031
BBa_K404253	.	sequence_alteration	2420	2422	.	+	0	ID=annotation2101880;Name=HSPG-KO
BBa_K404253	.	promoter	2	655	.	+	0	ID=annotation2094653;Name=CMV
BBa_K404253	.	sequence_feature	1274	2908	.	+	0	ID=annotation2094656;Name=VP3
BBa_K404253	.	sequence_feature	2435	2452	.	+	0	ID=annotation2094658;Name=His-tag
BBa_K404253	.	sequence_feature	2429	2434	.	+	0	ID=annotation2094659;Name=TG-spacer
BBa_K404253	.	sequence_alteration	2454	2456	.	+	0	ID=annotation2101879;Name=HSPG-KO
BBa_K404253	.	sequence_feature	671	3031	.	+	0	ID=annotation2094657;Name=AAV-2-cap (Stratagene)
BBa_K404253	.	sequence_feature	671	2908	.	+	0	ID=annotation2094654;Name=VP1
BBa_K404253	.	sequence_feature	2453	2461	.	+	0	ID=annotation2094660;Name=GLS-spacer
BBa_K404253	.	sequence_feature	2462	2477	.	+	0	ID=annotation2094662;Name=downstream 587
BBa_K404253	.	sequence_feature	2397	2428	.	+	0	ID=annotation2094661;Name=upstream 587
BBa_K404253	.	sequence_feature	1079	2908	.	+	0	ID=annotation2094655;Name=VP2
>BBa_K404253
gcgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagt
tcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgc
ccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatt
tacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaa
atggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatc
gctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtc
tccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgc
cccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaaccc
actgcttactggcttatcgaaattactagatggccggcgctgccgatggttatcttccagattggctcgaggacactct
ctctgaaggaataagacagtggtggaagctcaaacctggcccaccaccaccaaagcccgcagagcggcataaggacgac
agcaggggtcttgtgcttcctgggtacaagtacctcggacccttcaacggactcgacaagggagagccggtcaacgagg
cagacgccgcggccctcgagcacgacaaagcctacgaccggcagctcgacagcggagacaacccgtacctcaagtacaa
ccacgccgacgcggagtttcaggagcgccttaaagaagatacgtcttttgggggcaacctcggacgagcagtcttccag
gcgaaaaagagggttcttgaacctctgggcctggttgaggaacctgttaagacggctccgggaaaaaagaggccggtag
agcactctcctgtggagccagactcctcctcgggaaccggaaaggcgggccagcagcctgcaagaaaaagattgaattt
tggtcagactggagacgcagactcagtacctgacccccagcctctcggacagccaccagcagccccctctggtctggga
actaatacgatggctacaggcagtggcgcaccaatggcagacaataacgagggcgccgacggagtgggtaattcctcgg
gaaattggcattgcgattccacatggatgggcgacagagtcatcaccaccagcacccgaacctgggccctgcccaccta
caacaaccacctctacaaacaaatttccagccaatcaggagcctcgaacgacaatcactactttggctacagcacccct
tgggggtattttgacttcaacagattccactgccacttttcaccacgtgactggcaaagactcatcaacaacaactggg
gattccgacccaagagactcaacttcaagctctttaacattcaagtcaaagaggtcacgcagaatgacggtacgacgac
gattgccaataaccttaccagcacggttcaggtgtttactgactcggagtaccagctcccgtacgtcctcggctcggcg
catcaaggatgcctcccgccgttcccagcagacgtcttcatggtgccacagtatggatacctcaccctgaacaacggga
gtcaggcagtaggacgctcttcattttactgcctggagtactttccttctcagatgctgcgtaccggaaacaactttac
cttcagctacacttttgaggacgttcctttccacagcagctacgctcacagccagagtctggaccgtctcatgaatcct
ctcatcgaccaatatttgtattacttgagcagaacaaacactccaagtggtaccaccacgcagagtcgacttcagtttt
ctcaggccggagcgagtgacattcgggaccagtctaggaactggcttcctggaccctgttaccgccagcagcgagtatc
aaagacatctgcggataacaacaacagtgaatactcgtggactggagctaccaagtaccacctcaatggcagagactct
ctggtgaatccgggcccggccatggcaagccacaaggacgatgaagaaaagttttttcctcagagcggggttctcatct
ttgggaagcaaggctcagagaaaacaaatgtggacattgaaaaggtcatgattacagacgaagaggaaatcaggacaac
caatcccgtggctacggagcagtatggatccgtatctaccaacctccaggctggcaacaccggccatcatcatcatcat
catggcctgagcgcccaagcagctacagctgatgtcaacacacaaggcgttcttccaggcatggtctggcaggacagag
atgtgtaccttcaggggcccatctgggcaaagattccacacacggacggacattttcacccctctcccctcatgggtgg
attcggacttaaacaccctcctccacagattctcatcaagaacaccccggtacctgcgaatccttcgaccaccttcagt
gcggcaaagtttgcttccttcatcacacagtactccacgggacaggtcagcgtggagatcgagtgggagctccagaagg
aaaacagcaaacgctggaatcccgaaattcagtacacttccaactacaacaagtctgttaatgtggactttactgtgga
cactaatggcgtgtattcagagcctcgccccattggcaccagatacctgactcgtaatctgtaattgcttgttaatcaa
taaaccgtttaattcgtttcagttgaactttggtctctgcgtatttctttcttatctagtttccatggctacgtagata
agtagcatggcgggttaatcattaactac