##gff-version 3
##sequence-region BBa_K381001 1 986
BBa_K381001	.	terminator	858	937	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K381001	.	engineered_region	858	937	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K381001	.	stem_loop	869	912	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K381001	.	promoter	1	100	.	+	0	ID=BBa_K216005;Name=BBa_K216005
BBa_K381001	.	non_covalent_binding_site	50	65	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
BBa_K381001	.	non_covalent_binding_site	58	80	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
BBa_K381001	.	non_covalent_binding_site	66	71	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K381001	.	non_covalent_binding_site	89	94	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
BBa_K381001	.	CDS	130	849	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K381001	.	CDS	130	849	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K381001	.	ribosome_entry_site	109	123	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K381001	.	ribosome_entry_site	116	120	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K381001	.	engineered_region	109	123	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K381001	.	ribosome_entry_site	109	123	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K381001	.	terminator	946	986	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K381001	.	stop_codon	979	979	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K381001	.	polyA_site	973	986	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K381001	.	stem_loop	953	972	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K381001	.	engineered_region	946	986	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K381001
ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaat
tatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata