##gff-version 3
##sequence-region BBa_K360125 1 1928
BBa_K360125	.	engineered_region	58	1928	.	+	0	ID=BBa_K360024;Name=BBa_K360024
BBa_K360125	.	engineered_region	58	987	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_K360024
BBa_K360125	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K360125	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K360125	.	terminator	947	987	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K360125	.	stem_loop	954	973	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K360125	.	polyA_site	974	987	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K360125	.	stop_codon	980	980	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K360125	.	engineered_region	947	987	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K360125	.	terminator	859	938	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K360125	.	stem_loop	870	913	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K360125	.	engineered_region	859	938	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K360125	.	CDS	76	825	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K360125	.	engineered_tag	826	850	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K360125	.	CDS	79	786	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K360125	.	tag	787	819	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K360125	.	engineered_region	996	1928	.	+	0	ID=BBa_K098991;Name=BBa_K098991;Parent=BBa_K360024
BBa_K360125	.	terminator	1888	1928	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K098991
BBa_K360125	.	stem_loop	1895	1914	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K360125	.	polyA_site	1915	1928	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K360125	.	stop_codon	1921	1921	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K360125	.	engineered_region	1888	1928	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K360125	.	ribosome_entry_site	1053	1065	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K098991
BBa_K360125	.	engineered_region	1053	1065	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K360125	.	ribosome_entry_site	1059	1062	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K360125	.	ribosome_entry_site	1053	1065	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K360125	.	CDS	1072	1791	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K098991
BBa_K360125	.	CDS	1072	1791	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K360125	.	promoter	996	1044	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K098991
BBa_K360125	.	engineered_region	996	1044	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K360125	.	non_covalent_binding_site	1020	1036	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K360125	.	promoter	1033	1038	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K360125	.	non_covalent_binding_site	996	1012	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K360125	.	promoter	1010	1015	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K360125	.	terminator	1800	1879	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K098991
BBa_K360125	.	stem_loop	1811	1854	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K360125	.	engineered_region	1800	1879	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K360125	.	promoter	1	49	.	+	0	ID=BBa_K360023;Name=BBa_K360023
BBa_K360125	.	non_covalent_binding_site	29	45	.	+	0	ID=annotation2091493;Name=trpR binding site -3.5;Parent=BBa_K360023
BBa_K360125	.	promoter	28	35	.	+	0	ID=annotation2091497;Name=-10;Parent=BBa_K360023
BBa_K360125	.	promoter	3	11	.	+	0	ID=annotation2091496;Name=-35 ;Parent=BBa_K360023
BBa_K360125	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation2091495;Name=trpR binding site  -19.5;Parent=BBa_K360023
BBa_K360125	.	non_covalent_binding_site	21	40	.	+	0	ID=annotation2091494;Name=trpR binding site  -11.5;Parent=BBa_K360023
>BBa_K360125
gctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtactagagaaagaggagaaatactagatga
gcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaa
tgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggc
atcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaa
tcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgtttt
ttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcaca
accaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaa
gctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgg
gggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtaccca
atgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctg
caaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctg
gcggtgataatggttgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata